{"title":"Diversity and Aggressiveness of the <i>Diaporthe</i> Species Complex on Sunflower in Serbia.","authors":"Slobodan Krsmanović, Luca Riccioni, Boško Dedić, Febina Merlin Mathew, Miodrag Tolimir, Vera Stojšin, Kristina Petrović","doi":"10.1094/PDIS-01-24-0195-RE","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate the <i>Diaporthe</i> species associated with Phomopsis stem canker of sunflower (<i>Helianthus annuus</i> L.) in Serbia. The significant increase in sunflower and soybean (<i>Glycine max</i> [L.] Merr.) cultivation may have created the bridge favorable conditions for the distribution of <i>Diaporthe</i> species in this region. The present study identified five <i>Diaporthe</i> species on sunflower: <i>D. gulyae</i>, <i>D. helianthi</i>, <i>D. pseudolongicolla</i>, <i>D. stewartii</i>, and the newly identified <i>D. riccionae</i> based on morphological, molecular, and pathogenic characteristics. The research emphasizes the importance of effective inoculation methods and evaluates the aggressiveness of isolates. Sunflower plants were inoculated using the stem wound method, while seeds of sunflower and soybean were inoculated using the standard seed method. Most of the tested isolates demonstrated high aggressiveness, resulting in more than 80% premature wilting of sunflower plants. Additionally, this research examined the aggressiveness of <i>Diaporthe</i> species on sunflower seeds, highlighting <i>D. stewartii</i> and <i>D. pseudolongicolla</i> as common pathogens of both sunflower and soybean. The most aggressive species on seeds was <i>D. stewartii</i>, causing seed decay of up to 100% in sunflower and 97% in soybean. The findings suggest the development of resilient sunflower genotypes through breeding programs and the implementation of strategies to manage cross-contamination risks between sunflower and soybean crops. Furthermore, this study provides insights into the interactions between <i>Diaporthe</i> species and the seeds of sunflower and soybean. Future research will enhance our understanding of the impact of <i>Diaporthe</i> species on sunflower and soybean.</p>","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PDIS-01-24-0195-RE","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to investigate the Diaporthe species associated with Phomopsis stem canker of sunflower (Helianthus annuus L.) in Serbia. The significant increase in sunflower and soybean (Glycine max [L.] Merr.) cultivation may have created the bridge favorable conditions for the distribution of Diaporthe species in this region. The present study identified five Diaporthe species on sunflower: D. gulyae, D. helianthi, D. pseudolongicolla, D. stewartii, and the newly identified D. riccionae based on morphological, molecular, and pathogenic characteristics. The research emphasizes the importance of effective inoculation methods and evaluates the aggressiveness of isolates. Sunflower plants were inoculated using the stem wound method, while seeds of sunflower and soybean were inoculated using the standard seed method. Most of the tested isolates demonstrated high aggressiveness, resulting in more than 80% premature wilting of sunflower plants. Additionally, this research examined the aggressiveness of Diaporthe species on sunflower seeds, highlighting D. stewartii and D. pseudolongicolla as common pathogens of both sunflower and soybean. The most aggressive species on seeds was D. stewartii, causing seed decay of up to 100% in sunflower and 97% in soybean. The findings suggest the development of resilient sunflower genotypes through breeding programs and the implementation of strategies to manage cross-contamination risks between sunflower and soybean crops. Furthermore, this study provides insights into the interactions between Diaporthe species and the seeds of sunflower and soybean. Future research will enhance our understanding of the impact of Diaporthe species on sunflower and soybean.
期刊介绍:
Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.