Enhanced burn wound healing by controlled-release 3D ADMSC-derived exosome-loaded hyaluronan hydrogel.

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-03-26 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae035
Delong Zhu, Ying Hu, Xiangkai Kong, Yuansen Luo, Yi Zhang, Yu Wu, Jiameng Tan, Jianwei Chen, Tao Xu, Lei Zhu
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引用次数: 0

Abstract

Adipose mesenchymal stem cell (ADMSC)-derived exosomes (ADMSC-Exos) have shown great potential in regenerative medicine and been evidenced benefiting wound repair such as burns. However, the low yield, easy loss after direct coating, and no suitable loading system to improve their availability and efficacy hinder their clinical application for wound healing. And few studies focused on the comparison of biological functions between exosomes derived from different culture techniques, especially in exosome-releasing hydrogel system. Therefore, we designed a high-performance exosome controllable releasing hydrogel system for burn wound healing, namely loading 3D-printed microfiber culture-derived exosomes in a highly biocompatible hyaluronic acid (HA). In this project, we compared the biological functions in vitro and in a burn model among exosomes derived from the conventional two-dimensional (2D) plate culture (2D-Exos), microcarrier culture (2.5D-Exos), and 3D-printed microfiber culture (3D-Exos). Results showed that compared with 2D-Exos and 2.5D-Exos, 3D-Exos promoted HACATs and HUVECs cell proliferation and migration more significantly. Additionally, 3D-Exos had stronger angiogenesis-promoting effects in tube formation of (HUVECs) cells. Moreover, we found HA-loaded 3D-Exos showed better burn wound healing promotion compared to 2D-Exos and 2.5D-Exos, including accelerated burn wound healing rate and better collagen remodeling. The study findings reveal that the HA-loaded, controllable-release 3D-Exos repair system distinctly augments therapeutic efficacy in terms of wound healing, while concurrently introducing a facile application approach. This system markedly bolsters the exosomal loading efficiency, provides a robust protective milieu, and potentiates the inherent biological functionalities of the exosomes. Our findings provide a rationale for more efficient utilization of high-quality and high-yield 3D exosomes in the future, and a novel strategy for healing severe burns.

通过控制释放三维 ADMSC 衍生的外泌体载荷透明质酸水凝胶促进烧伤创面愈合。
脂肪间充质干细胞(ADMSC)衍生的外泌体(ADMSC-Exos)在再生医学中显示出巨大的潜力,并被证明有利于烧伤等伤口的修复。然而,外泌体的产量低、直接包被后容易流失,而且没有合适的负载系统来提高其可用性和功效,这些都阻碍了其在伤口愈合方面的临床应用。此外,很少有研究关注不同培养技术获得的外泌体之间生物功能的比较,尤其是在外泌体释放水凝胶系统中。因此,我们设计了一种用于烧伤创面愈合的高性能外泌体可控释放水凝胶系统,即在高生物相容性的透明质酸(HA)中加载三维打印微纤维培养衍生的外泌体。在该项目中,我们比较了传统二维平板培养(2D-Exos)、微载体培养(2.5D-Exos)和三维打印微纤维培养(3D-Exos)获得的外泌体在体外和烧伤模型中的生物功能。结果表明,与 2D-Exos 和 2.5D-Exos 相比,3D-Exos 对 HACATs 和 HUVECs 细胞增殖和迁移的促进作用更为显著。此外,3D-Exos对(HUVECs)细胞的血管形成具有更强的促进作用。此外,我们发现与 2D-Exos 和 2.5D-Exos 相比,HA 负载的 3D-Exos 对烧伤创面愈合有更好的促进作用,包括加快烧伤创面愈合速度和改善胶原重塑。研究结果表明,负载 HA 的可控释放 3D-Exos 修复系统明显提高了伤口愈合的疗效,同时还采用了简便的应用方法。该系统显著提高了外泌体的装载效率,提供了稳健的保护环境,并增强了外泌体固有的生物功能。我们的研究结果为今后更有效地利用高质量、高产出的三维外泌体提供了理论依据,也为治愈严重烧伤提供了一种新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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