Stoichiometry of long noncoding RNA interactions with other RNAs: Insights from OIP5-AS1.

IF 6.4 2区 生物学 Q1 CELL BIOLOGY
Jen-Hao Yang, Dimitrios Tsitsipatis, Myriam Gorospe
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引用次数: 0

Abstract

Long noncoding (lnc)RNAs modulate gene expression programs in a range of developmental processes in different organs. In skeletal muscle, lncRNAs have been implicated in myogenesis, the process whereby muscle precursor cells form muscle fibers during embryonic development and regenerate muscle fibers in the adult. Here, we discuss OIP5-AS1, a lncRNA that is highly expressed in skeletal muscle and is capable of coordinating protein expression programs during myogenesis. Given that several myogenic functions of OIP5-AS1 involve interactions with MEF2C mRNA and with the microRNA miR-7, it was critical to carefully evaluate the precise levels of OIP5-AS1 during myogenesis. We discuss the approaches used to examine lncRNA copy number using OIP5-AS1 as an example, focusing on quantification by quantitative PCR analysis with reference to nucleic acids of known abundance, by droplet digital (dd)PCR measurement, and by microscopic visualization of individual lncRNAs in cells. We discuss considerations of RNA stoichiometry in light of developmental processes in which lncRNAs are implicated. This article is categorized under: Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs.

长非编码 RNA 与其他 RNA 相互作用的计量:来自 OIP5-AS1 的启示
长非编码(lnc)RNA 在不同器官的一系列发育过程中调节基因表达程序。在骨骼肌中,lncRNA 与肌生成有关,肌生成是肌肉前体细胞在胚胎发育过程中形成肌纤维并在成年后再生肌纤维的过程。在这里,我们讨论了 OIP5-AS1,它是一种在骨骼肌中高表达的 lncRNA,能够在肌生成过程中协调蛋白质表达程序。鉴于OIP5-AS1的几种肌生成功能涉及与MEF2C mRNA和microRNA miR-7的相互作用,因此仔细评估肌生成过程中OIP5-AS1的精确水平至关重要。我们以OIP5-AS1为例,讨论了用于检测lncRNA拷贝数的方法,重点是参照已知丰度的核酸进行定量PCR分析、液滴数字(dd)PCR测量以及细胞中单个lncRNA的显微可视化。我们根据与 lncRNAs 有关的发育过程,讨论了对 RNA 平衡度的考虑。本文归类于调控 RNAs/RNAi/Riboswitches > 调控 RNAs。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
14.80
自引率
4.10%
发文量
67
审稿时长
6-12 weeks
期刊介绍: WIREs RNA aims to provide comprehensive, up-to-date, and coherent coverage of this interesting and growing field, providing a framework for both RNA experts and interdisciplinary researchers to not only gain perspective in areas of RNA biology, but to generate new insights and applications as well. Major topics to be covered are: RNA Structure and Dynamics; RNA Evolution and Genomics; RNA-Based Catalysis; RNA Interactions with Proteins and Other Molecules; Translation; RNA Processing; RNA Export/Localization; RNA Turnover and Surveillance; Regulatory RNAs/RNAi/Riboswitches; RNA in Disease and Development; and RNA Methods.
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