Effects of CDDO-EA in sepsis-induced acute lung injury: mouse model of endotoxaemia.

Q4 Medicine

Wiadomosci lekarskie Pub Date : 2024-01-01 DOI:10.36740/WLek202403119

Mohammed Hamzah Ibadi, Sahar Majeed, Fadhaa Abdulameer Ghafil, Najah R Hadi

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引用次数: 0

Abstract

Objective: Aim: The aim of this research is to clarify the potential effect of CDDO-EA against experimentally sepsis induced lung injury in mice.

Patients and methods: Materials and Methods: Mice have divided into four groups: Sham group CLP group, Vehicle-treatment group, CDDO-EA-treated group: mice in this group received CDDO-EA 2mg/kg intraperitoneally, 1hr before CLP, then the animals were sacrificed 24hr after CLP. After exsAngpuinations, tissue samples of lung were collected, followed by markers measurement including, TNF-α, IL-1β, VEGF, MPO, caspase11, Angp-1and Angp-2 by ELISA, gene expression of TIE2 and VE-cadherin by qRT-PCR, in addition to histopathological study.

Results: Results: A significant elevation (p<0.05) in TNF-α, IL-1β, MPO, ANGP-2, VEGF, CASPASE 11 in CLP and vehicle groups when compared with sham group. CDDO-EA group showed significantly lower levels p<0.05, level of ANGP-1 was significantly lower p<0.05 in the CLP and vehicle groups as compared with the sham group. Quantitative real-time PCR demonstrated a significant decrement in mRNA expression of TIE2&ve-cadherin genes p<0.05 in sepsis & vehicle.

Conclusion: Conclusions: CDDO-EA has lung protective effects due to its anti-inflammatory and antiAngpiogenic activity, additionally, CDDO-EA showes a lung protective effect as they affect tissue mRNA expression of TIE2 and cadherin gene. Furthermore, CDDO-EA attenuate the histopathological changes that occur during polymicrobial sepsis thereby lung protection effect.

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CDDO-EA 对败血症诱发的急性肺损伤的影响：小鼠内毒素血症模型。

研究目的目的：本研究旨在阐明 CDDO-EA 对实验性败血症诱导的小鼠肺损伤的潜在作用：材料与方法：小鼠分为四组：Sham 组 CLP 组，Vehicle-treatment 组，CDDO-EA-treated 组：本组小鼠在 CLP 前 1 小时腹腔注射 CDDO-EA 2mg/kg，CLP 后 24 小时处死。去势后采集肺组织样本，采用 ELISA 法检测 TNF-α、IL-1β、VEGF、MPO、caspase11、Angp-1 和 Angp-2，采用 qRT-PCR 法检测 TIE2 和 VE-cadherin 的基因表达，并进行组织病理学研究：结果：结果：结果：结果表明，血管内皮生长因子（TIE2）和血管粘连蛋白（VEadherin）的基因表达明显增加（p结论CDDO-EA 具有抗炎和抗血管生成活性，因此对肺部有保护作用；此外，CDDO-EA 还影响组织中 TIE2 和干酪素基因 mRNA 的表达，因此对肺部有保护作用。此外，CDDO-EA 还能减轻多微生物败血症时出现的组织病理学变化，从而起到保护肺部的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Wiadomosci lekarskie Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

482