Analysis of flow cytometry data from ultrasound-guided lymph node biopsies with two types of needles

IF 2.2 4区 医学 Q3 HEMATOLOGY
Xin Jin, Huifang Jiang, Yuxia Jiang, Zhilu Chen, Wenfei Zhou, Qing Pan, Shuyuan Tian
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引用次数: 0

Abstract

Background

In this study, we combined two techniques, ultrasound-guided needle biopsy and flow cytometry (FCM), to explore their value in patients with enlarged lymph nodes.

Methods

We compared the results of 198 needle biopsies on FCM and pathology. Forty-two were done by (fine needle aspiration, FNA), and the remaining 156 with (core needle biopsy, CNB), in 36 of 156 patients, a FNA was performed in the same lymph node after completion of the CNB. Except for five types of pathological entities, the rest were differentiated only detected or undetected tumours as the outcome distinction.

Results

Among the 198 needle biopsies, 13 were inadequate specimens, while the remaining 185 had pathological findings, including 47 benign and 138 neoplastic findings. Thirty-six patients underwent puncture with both FNA and CNB, both needles produced identical results by FCM, but more cells were obtained by FNA. Among the pathologically positive results, there were 23 missed diagnoses in FCM, in contrast, evidence of tumours was observed in the FCM images of 15 needle biopsies that reported benign or findings that were inconsistent with pathology, and the final diagnosis was consistent with the FCM in 10 cases. FCM detected haematolymphoid tumours with a sensitivity of 87.8% and a specificity of 91.9%.

Conclusion

The combination of FCM and ultrasound-guided lymph node needle biopsy can quickly provide guidance for clinical decision-making. We recommend that all lymph node needle biopsies be sent for FCM, the specimen can be obtained by the last puncture with FNA.

使用两种针头对超声引导下的淋巴结活检进行流式细胞术数据分析。
背景:在这项研究中,我们将超声引导针刺活检和流式细胞术(FCM)这两种技术结合起来,探讨它们在淋巴结肿大患者中的价值:在这项研究中,我们将超声引导下针穿活检和流式细胞术(FCM)这两种技术结合起来,探讨它们在淋巴结肿大患者中的应用价值:我们比较了 198 例针活检的 FCM 和病理结果。在 156 例患者中,有 36 例在完成 CNB 后在同一淋巴结进行了 FNA。除五种病理实体外,其余仅以检测到或未检测到肿瘤作为结果区分:结果:在 198 例针穿活检中,13 例标本不足,其余 185 例均有病理结果,包括 47 例良性肿瘤和 138 例肿瘤。36 名患者同时接受了 FNA 和 CNB 穿刺,两种穿刺针的 FCM 结果相同,但 FNA 获得的细胞更多。在病理阳性结果中,有 23 例在 FCM 中漏诊,相反,在 15 例报告为良性或与病理结果不一致的针刺活检的 FCM 图像中观察到了肿瘤的证据,有 10 例的最终诊断与 FCM 一致。FCM 检测血淋巴肿瘤的灵敏度为 87.8%,特异度为 91.9%:结论:结合 FCM 和超声引导下淋巴结穿刺活检可快速为临床决策提供指导。我们建议将所有淋巴结穿刺活检送去进行 FCM,标本可通过 FNA 最后一次穿刺获得。
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来源期刊
CiteScore
4.50
自引率
6.70%
发文量
211
审稿时长
6-12 weeks
期刊介绍: The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.
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