Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-07-01 Epub Date: 2024-05-03 DOI:10.1007/s12275-024-00136-x

Woo Young Kim, Seung-Jin Kim, Hye-Rin Seo, Yoonyong Yang, Jong Seok Lee, Moonsuk Hur, Byoung-Hee Lee, Jong-Geol Kim, Min-Kyu Oh

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Abstract

Production of medium chain length polyhydroxyalkanoate (mcl-PHA) was attempted using Pseudomonas gessardii NIBRBAC000509957, which was isolated from Sunchang, Jeollabuk-do, Republic of Korea (35°24'27.7"N, 127°09'13.0"E) and effectively utilized acetate and formate as carbon sources. We first evaluated the utilization of acetate as a carbon source, revealing optimal growth at 5 g/L acetate. Then, formate was supplied to the acetate minimal medium as a carbon source to enhance cell growth. After overexpressing the acetate and formate assimilation pathway enzymes, this strain grew at a significantly higher rate in the medium. As this strain naturally produces PHA, it was further engineered metabolically to enhance mcl-PHA production. The engineered strain produced 0.40 g/L of mcl-PHA with a biomass content of 30.43% in fed-batch fermentation. Overall, this strain can be further developed to convert acetate and formate into valuable products.

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以醋酸-甲酸酯为碳源的工程假单胞菌生产中链长度聚羟基烷酸酯

该假单胞菌分离自大韩民国全罗北道顺昌（35°24'27.7 "N，127°09'13.0 "E），能有效利用醋酸盐和甲酸盐作为碳源。我们首先评估了醋酸盐作为碳源的利用情况，结果表明在 5 克/升醋酸盐条件下生长最佳。然后，在乙酸最小培养基中加入甲酸盐作为碳源，以促进细胞生长。在过表达乙酸和甲酸同化途径酶后，该菌株在培养基中的生长速度明显提高。由于该菌株能自然产生 PHA，因此对其进行了进一步的代谢改造，以提高 mcl-PHA 的产量。经改造的菌株在饲料批量发酵过程中产生了 0.40 克/升的 mcl-PHA，生物量含量为 30.43%。总之，该菌株可进一步开发，将醋酸盐和甲酸盐转化为有价值的产品。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567