The StuA Transcription Factor and Alternative Splicing Mechanisms Drive the Levels of MAPK Hog1 Transcripts in the Dermatophyte Trichophyton rubrum.

IF 3.6 3区 生物学 Q2 MYCOLOGY
Leonardo Martins-Santana, Monise Fazolin Petrucelli, Pablo R Sanches, Fausto Almeida, Nilce M Martinez-Rossi, Antonio Rossi
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Abstract

Trichophyton rubrum is a human fungal pathogen that causes dermatophytosis, an infection that affects keratinized tissues. Integrated molecular signals coordinate mechanisms that control pathogenicity. Transcriptional regulation is a core regulation of relevant fungal processes. Previous RNA sequencing data revealed that the absence of the transcription factor StuA resulted in the differential expression of the MAPK-related high glycerol osmolarity gene (hog1) in T. rubrum. Here we validated the role of StuA in regulating the transcript levels of hog1. We showed through RT-qPCR that transcriptional regulation controls hog1 levels in response to glucose, keratin, and co-culture with human keratinocytes. In addition, we also detected hog1 pre-mRNA transcripts that underwent alternative splicing, presenting intron retention in a StuA-dependent mechanism. Our findings suggest that StuA and alternative splicing simultaneously, but not dependently, coordinate hog1 transcript levels in T. rubrum. As a means of preventing and treating dermatophytosis, our results contribute to the search for new potential drug therapies based on the molecular aspects of signaling pathways in T. rubrum.

Abstract Image

StuA转录因子和替代剪接机制驱动皮癣菌中 MAPK Hog1 转录本的水平。
红色毛癣菌(Trichophyton rubrum)是一种人类真菌病原体,可引起皮肤癣菌病(一种影响角质化组织的感染)。综合分子信号协调了控制致病性的机制。转录调控是相关真菌过程的核心调控机制。之前的 RNA 测序数据显示,转录因子 StuA 的缺失会导致红癣菌中与 MAPK 相关的高甘油渗透压基因(hog1)的差异表达。在这里,我们验证了 StuA 在调节 hog1 转录本水平中的作用。我们通过 RT-qPCR 发现,转录调控控制着 hog1 对葡萄糖、角蛋白以及与人类角质细胞共培养的反应水平。此外,我们还检测到 hog1 前 mRNA 转录本进行了替代剪接,在 StuA 依赖性机制中出现了内含子保留。我们的研究结果表明,StuA 和替代剪接同时(但不是依赖性的)协调了红念珠菌中 hog1 的转录水平。作为预防和治疗皮肤癣菌病的一种手段,我们的研究结果有助于根据红念珠菌信号通路的分子方面寻找新的潜在药物疗法。
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来源期刊
Mycopathologia
Mycopathologia 生物-真菌学
CiteScore
6.80
自引率
3.60%
发文量
76
审稿时长
3 months
期刊介绍: Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.
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