Pangenome analyses of Clostridium butyricum provide insights into its genetic characteristics and industrial application

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Yicheng Yang , Yuan Shao , Chenchen Pei , Yangyang Liu , Min Zhang , Xi Zhu , Jinshan Li , Lifei Feng , Guanghua Li , Keke Li , Yunxiang Liang , Yingjun Li
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引用次数: 0

Abstract

Clostridium butyricum is a Gram-positive anaerobic bacterium known for its ability to produce butyate. In this study, we conducted whole-genome sequencing and assembly of 14C. butyricum industrial strains collected from various parts of China. We performed a pan-genome comparative analysis of the 14 assembled strains and 139 strains downloaded from NCBI. We found that the genes related to critical industrial production pathways were primarily present in the core and soft-core gene categories. The phylogenetic analysis revealed that strains from the same clade of the phylogenetic tree possessed similar antibiotic resistance and virulence factors, with most of these genes present in the shell and cloud gene categories. Finally, we predicted the genes producing bacteriocins and botulinum toxins as well as CRISPR systems responsible for host defense. In conclusion, our research provides a desirable pan-genome database for the industrial production, food application, and genetic research of C. butyricum.

通过对丁酸梭菌的庞基因组分析,可以深入了解其遗传特征和工业应用。
丁酸梭菌(Clostridium butyricum)是一种革兰氏阳性厌氧细菌,以其生产丁酸盐的能力而闻名。在这项研究中,我们对从中国各地收集到的 14 株丁酸梭菌工业菌株进行了全基因组测序和组装。我们对 14 个组装菌株和从 NCBI 下载的 139 个菌株进行了泛基因组比较分析。我们发现,与关键工业生产途径相关的基因主要存在于核心基因和软核基因类别中。系统进化分析表明,系统进化树上同一支系的菌株具有相似的抗生素耐药性和毒力因子,这些基因大多存在于外壳和云基因类别中。最后,我们预测了产生细菌素和肉毒杆菌毒素的基因以及负责宿主防御的 CRISPR 系统。总之,我们的研究为丁酸杆菌的工业生产、食品应用和基因研究提供了一个理想的泛基因组数据库。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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