The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Mediates the Inflammatory Process in Deep Dermal Burn Injury.

Abstract

The combination of platelet-rich plasma (PRP) and stromal vascular fraction cells (SVFs) was beneficial in accelerating wound healing. This study aims to assess the effect of this combination in balancing the inflammatory process to accelerate burn healing. Thirty eligible Wistar rats were used in this study to establish a deep dermal degree burn wound model. They were randomly divided into four groups: locally injected with the combination of SVFs and PRP (n=9), vaseline (n=9), placebo (n=9), and healthy Wistar rats group (n=3), as treatment group, positive control group, negative control group and healthy control group, respectively. The burn wound tissue was excised from three separated sacrificed rats (8, 24 and 48 hours) to examine polymorphonuclear (PMN) and lymphocyte counts through the standard hematoxylin-eosin procedure and for cyclooxygenase2 (COX-2) expression through the immunohistochemical procedure. The highest PMN, lymphocyte cell count, and COX 2 expression were found at 8 hours in the local injection with the PRP combination SVF group (28,555±11,237, 8,111±3,218, and 4,666±2,309, respectively, p <0.05 except for COX 2). The regression analysis results showed that local injection of a combination of PRP and SVF could reduce PMN cells by 1.068 times, lymphocytes by 1.786 times, and COX 2 by 1.853 times greater than topical application with vaseline. The combined injection of PRP and SVF effectively heals deep burns by acutely increasing the PMN cell and lymphocyte count, and COX 2 expression. Conversely, the treatment decreased the PMN cell and lymphocyte count but not the COX 2 expression in the sub-acute phase of wound healing.