Sodium Sulfite as a Novel Hypoxia Revulsant Involved in Hypoxic Regulation in Escherichia coli.

Qiao Ye, Jia Nan Huo, Yuan Luo, Zhu Song Mei, Long Mei Fang, Bing Qian Guo, Guang Yun Wang
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Abstract

As a reducing salt, sodium sulfite could deprive oxygen in solution, which could mimic hypoxic stress in Caenorhabditis elegans. In this study, the wild-type Escherichia coli strain MG1655 was used to examine the inhibition of sodium sulfite-induced hypoxia by observing the bacterial growth curves. We also analyzed the growth curves of mutant strains (for arcA/B, soxR/S, fnr, and oxyR) related to E. coli hypoxic pathways to reveal roles of the related genes during hypoxia. The ultrastructure of hypoxia-inhibited bacteria were also observed using transmission electron microscopy. Sodium sulfite could maintain hypoxic condition of bacterial culture for 8 h with concentrations over 40 mmol/L. Complete ultrastructure of the bacteria indicated sodium sulfite did inhibit bacterial growth and division. Among the hypoxia genes, fnr and arcB played key roles in sodium sulfite-induced hypoxia. This study showed that sodium sulfite could be used as a novel hypoxia revulsant for bacterial cultures.

亚硫酸钠是一种参与大肠杆菌缺氧调节的新型缺氧振奋剂
亚硫酸钠作为一种还原盐,可使溶液中缺氧,从而模拟草履虫的缺氧应激。本研究使用野生型大肠杆菌菌株 MG1655,通过观察细菌的生长曲线来研究亚硫酸钠诱导缺氧的抑制作用。我们还分析了与大肠杆菌缺氧通路相关的突变株(arcA/B、soxR/S、fnr 和 oxyR)的生长曲线,以揭示相关基因在缺氧过程中的作用。此外,还利用透射电子显微镜观察了缺氧抑制细菌的超微结构。亚硫酸钠浓度超过 40 mmol/L 时,可维持细菌培养 8 小时的缺氧状态。细菌的完整超微结构表明亚硫酸钠确实抑制了细菌的生长和分裂。在缺氧基因中,fnr 和 arcB 在亚硫酸钠诱导的缺氧中起关键作用。这项研究表明,亚硫酸钠可作为一种新型的缺氧抑制剂用于细菌培养。
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