Serum exosome tRFs as a promising biomarker for active tuberculosis and latent tuberculosis infection

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2024-05-04 DOI:10.1016/j.mimet.2024.106944

Xiangyu Xi , Binghua Wang , Ruimei Zhang , Chunhua Ling

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引用次数: 0

Abstract

Objective

To analyse the expression profiles of serum exosome tRFs/tiRNAs and to explore their diagnostic value in tuberculosis (TB) activity.

Methods

The serum exosome tRF/tiRNA profile was analysed using high-throughput sequencing technology in 5 active tuberculosis (ATB) patients, 5 latent tuberculosis infection (LTBI) patients and 5 healthy controls (HCs). Then, serum exosome tRFs/tiRNAs were validated by quantitative real-time polymerase chain reaction (qRT–PCR), and their diagnostic value was evaluated by receiver operating characteristic curve (ROC) and area under the curve (AUC). Finally, bioinformatics analysis was performed to explore and identify the potential biological pathways induced by tRFs/tiRNAs.

Results

The sequencing results revealed that serum exosome tRF/tiRNA expression profiles were different among ATB patients, LTBI patients and HCs. Three tRFs (tRF-56:75-Trp-CCA-4, tRF-1:22-chrM.Ser-GCT and tRF-56:76-Val-TAC-1-M2) were selected for qRT–PCR validation. The results demonstrated that the expression level of tRF-1-22-chrM.Ser-GCT was upregulated in ATB patients, while tRF-56-75-Trp-CCA-4 was downregulated, which was consistent with the sequencing data. The AUCs of tRF-56:75-Trp-CCA-4 and tRF-1:22-chrM. Ser-GCT were 0.824 and 1.000, respectively, which have significant values in the diagnosis of ATB patients. Moreover, the expression levels of tRF-56:75-Trp-CCA-4 and tRF-1:22-chrM.Ser-GCT and tRF-56:76-Val-TAC-1-M2 in ATB patients and LTBI were different, which indicated that these three tRFs could effectively distinguish ATB patients and LTBI patients.

Conclusion

Our findings indicate that serum exosome tRFs can be used as potential markers for the diagnosis of ATB and LTBI.

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血清外泌体 tRFs 作为活动性肺结核和潜伏性肺结核感染的有望生物标记物。

目的分析血清外泌体tRFs/tiRNA的表达谱，并探讨其在结核病（TB）活动中的诊断价值：采用高通量测序技术分析了5例活动性肺结核（ATB）患者、5例潜伏肺结核感染（LTBI）患者和5例健康对照（HCs）的血清外泌体tRF/tiRNA表达谱。然后，通过实时定量聚合酶链式反应（qRT-PCR）对血清外泌体 tRFs/tiRNAs 进行了验证，并通过接收者操作特征曲线（ROC）和曲线下面积（AUC）对其诊断价值进行了评估。最后，进行了生物信息学分析，以探索和确定tRFs/tiRNAs诱导的潜在生物通路：结果：测序结果显示，ATB 患者、LTBI 患者和 HCs 的血清外泌体 tRF/tiRNA 表达谱不同。结果：测序结果显示，ATB 患者、LTBI 患者和 HCs 的血清外泌体 tRF/tiRNA 表达谱不同。结果显示，tRF-1-22-chrM.Ser-GCT在ATB患者中表达水平上调，而tRF-56-75-Trp-CCA-4表达水平下调，这与测序数据一致。tRF-56:75-Trp-CCA-4和tRF-1:22-chrM.Ser-GCT的AUC为0.824。Ser-GCT的AUC分别为0.824和1.000，在ATB患者的诊断中具有重要价值。此外，tRF-56:75-Trp-CCA-4和tRF-1:22-chrM.Ser-GCT以及tRF-56:76-Val-TAC-1-M2在ATB患者和LTBI患者中的表达水平不同，这表明这三种tRFs能有效区分ATB患者和LTBI患者：我们的研究结果表明，血清外泌体 tRFs 可作为诊断 ATB 和 LTBI 的潜在标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.