Green synthesized AgNPs as a probe for colorimetric detection of Hg (II) ions in aqueous medium and fluorescent imaging in liver cell lines and its antibacterial activity.

Abstract

The present research aimed at green synthesis of Ag nanoparticles (AgNPs) based colorimetric sensor using persimmon leaf extract (PLE) for selective detection of mercuric ion (Hg²⁺). Optimization of reaction conditions viz. pH, concentration of PLE, time was done and further AgNPs were characterized using UV, IR, FE-SEM, EDX, XRD and TEM analysis. The developed AgNPs were evaluated for the selective colorimetric detection of Hg²⁺ in aqueous medium and fluorescence imaging of Hg²⁺ ions in liver cell lines. Later, the antibacterial activity of AgNPs was performed against S. aureus and E. coli. The findings of the study revealed that PLE mediated AgNPs exhibited notable limit of detection up to 0.1 ppb, high efficiency, and stability. The antibacterial study indicated that developed AgNPs has impressive bacterial inhibiting properties against the tested bacterial strains. In conclusion, developed biogenic AgNPs has high selectivity and notable sensitivity towards Hg²⁺ ions and may be used as key tool water remediation.