Seroepidemiology and associated risk factors of brucellosis in small ruminants of district Khanewal, Pakistan.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-12 eCollection Date: 2024-03-01 DOI:10.5455/javar.2024.k741

Abdul Sammad Ali Khan Shirwany, Mian Muhammad Awais, Muhammad Irfan Anwar, Muhammad Raza Hameed, Masood Akhtar, Nabeel Ijaz, Shakera Sadiq Gill, Muhammad Amjad Ali, Muhammad Sibtain Bhatti, Mamoona Chaudhry

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Abstract

Objectives: Keeping in view the economic and veterinary public health importance of brucellosis, this research was conducted to determine its seroprevalence and associated risk determinants in small ruminants in district Khanewal, Southern Punjab, Pakistan.

Materials and methods: Two-stage cluster sampling technique was used for sampling, and the sample size was calculated using C-survey 2.0. Accordingly, sera samples (n = 392) were collected from small ruminants in the study area from October 2022 to July 2023. All the samples were tested for the presence of anti-Brucella antibodies by Rose Bengal Plate Test (RBPT), followed by confirmation of all the samples using an enzyme linked immunosorbent assay (ELISA) kit (ID.vet®, France; sensitivity and specificity=100%, each).

Results: The seropositivity rate of brucellosis was 7.14% [n = 28/392; 95% confidence interval (CI) = 4.87%-10.12%] by RBPT, whereas the results of ELISA showed an overall seroprevalence rate of 7.40% (n = 29/392; 95% CI = 5.11%-10.37%) in the study population. Univariate analysis of risk factors revealed that abortion history (AH), retained fetal membranes (RFMs), repeat breeding, flock size (FS), educational status of farmers (ESFs), awareness about brucellosis (AB), and farm hygiene had a significant association with the seroprevalence of brucellosis (p < 0.05). The multivariate analysis using a binary logistic regression model revealed that variables including tehsil, FS, AH, RFM, ESF, AB, and farming system were significant factors (p < 0.05) associated with brucellosis in the target population.

Conclusion: Brucellosis is prevalent in small ruminants in Khanewal, Pakistan. The disease burden can be reduced by improving the reproductive health of animals, farm hygiene, and farmers' awareness about the diseases. Further studies are needed on a larger scale to devise stringent disease control strategies to avoid losses associated with brucellosis at regional, national, and global levels.

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巴基斯坦 Khanewal 地区小型反刍动物布鲁氏菌病的血清流行病学及相关风险因素。

研究目的：鉴于布鲁氏菌病在经济和兽医公共卫生方面的重要性，本研究旨在确定其在巴基斯坦南旁遮普省 Khanewal 地区小型反刍动物中的血清流行率和相关风险决定因素：采用两阶段聚类抽样技术进行抽样，并使用 C-survey 2.0 计算样本量。因此，在 2022 年 10 月至 2023 年 7 月期间，从研究地区的小反刍动物身上采集了血清样本（n = 392）。所有样本均通过玫瑰红平板试验（RBPT）检测是否存在抗布鲁氏杆菌抗体，然后使用酶联免疫吸附试验（ELISA）试剂盒（ID.vet®，法国；灵敏度和特异性均为100%）对所有样本进行确认：布鲁氏菌病的血清阳性率为 7.14% [n = 28/392；95% 置信区间 (CI) = 4.87%-10.12%]，而酶联免疫吸附测定的结果显示研究人群的总体血清阳性率为 7.40% (n = 29/392；95% CI = 5.11%-10.37%)。对风险因素的单变量分析表明，流产史（AH）、胎膜残留（RFM）、重复配种、鸡群规模（FS）、养殖户受教育程度（ESFs）、对布鲁氏菌病的认识（AB）和养殖场卫生与布鲁氏菌病的血清流行率有显著关系（P < 0.05）。使用二元逻辑回归模型进行的多变量分析表明，包括乡、FS、AH、RFM、ESF、AB 和耕作制度在内的变量与目标人群的布鲁氏菌病相关（p < 0.05）：结论：布鲁氏菌病在巴基斯坦 Khanewal 的小型反刍动物中很普遍。通过改善动物的生殖健康、农场卫生和提高农民对疾病的认识，可以减轻疾病负担。需要进行更大规模的进一步研究，以制定严格的疾病控制策略，避免布鲁氏菌病在地区、国家和全球层面造成损失。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.