Effect of electroacupuncture on proBDNF/mBDNF and synaptic plasticity in rats with learning and memory impairment after cerebral ischemia-reperfusion.

Q3 Medicine

针刺研究 Pub Date : 2024-04-25 DOI:10.13702/j.1000-0607.20221313

Ming-Yue Yu, Kai-Qi Su, Xin Shen, Huan-Huan Liu, Jing Gao, Jian Guo, Xiao-Dong Feng

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引用次数: 0

Abstract

Objectives: To observe the effect of electroacupuncture (EA) at "Baihui" (GV20) and "Shenting" (GV24) on the rats' behavior and the transforming precursor of brain-derived neurotrophic factor (proBDNF) into mature brain-derived neurotrophic factor (mBDNF) in the hippocampus of rats with learning and memory impairment induced by cerebral ischemia-reperfusion (IR), so as to explore its mechanisms underlying improvement of learning and memory ability.

Methods: SD rats were randomly divided into blank, sham operation, model, and EA groups, with 6 rats in each group. The model of IR was established by occlusion of the middle cerebral artery. EA (1 Hz/20 Hz) was applied to GV24 and GV20 for 30 min, once daily for 14 days. The neurological function was evaluated according to the Zea Longa's score criteria 24 h after modeling and after intervention. Morris water maze test was used to detect the learning and memory function of the rats. TTC staining was used to evaluate the cerebral infarction volume on the affected side. The protein expression levels of proBDNF, mBDNF, tissue plasminogen activator (tPA), tyrosine kinase receptor B (TrkB) and p75 neurotrophin receptor (p75NTR) in hippocampal tissue were detected by Western blot.

Results: Compared with the sham operation group, the neurological function score, the percentage of cerebral infarction volume and the expression levels of proBDNF and p75NTR protein in hippocampus were increased (P<0.01), while the times of crossing the original platform and the total distance in the target quadrant, the expression levels of mBDNF, TrkB and tPA protein and the ratio of mBDNF/proBDNF were decreased (P<0.01, P<0.05) in the model group. Compared with the model group, the neurological function score, the percentage of cerebral infarction volume, and the expression levels of proBDNF and p75NTR protein in hippocampus were decreased (P<0.01, P<0.05), while the times of crossing the original platform, the total distance in the target quadrant, and the expression levels of mBDNF, TrkB and tPA protein and the ratio of mBDNF/proBDNF were increased (P<0.05, P<0.01) in the EA group.

Conclusions: EA can alleviate learning and memory impairment in IR rats, which may be related to its function in up-regulating the expression of tPA protein and promoting the transformation of proBDNF to mBDNF, thus improving the synaptic plasticity.

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电针对脑缺血再灌注后学习记忆受损大鼠proBDNF/mBDNF和突触可塑性的影响

研究目的观察电针 "百会 "穴（GV20）和 "神庭 "穴（GV24）对脑缺血再灌注（IR）诱导的学习记忆障碍大鼠行为及脑源性神经营养因子前体（proBDNF）转化为成熟脑源性神经营养因子（mBDNF）的影响，探讨其改善学习记忆能力的机制：将 SD 大鼠随机分为空白组、假手术组、模型组和 EA 组，每组 6 只。通过闭塞大脑中动脉建立 IR 模型。在 GV24 和 GV20 上施加 EA（1 Hz/20 Hz）30 分钟，每天一次，连续 14 天。建模后 24 小时和干预后 24 小时，根据 Zea Longa 评分标准评估神经功能。莫里斯水迷宫测试用于检测大鼠的学习和记忆功能。TTC染色用于评估患侧脑梗死体积。Western blot检测海马组织中proBDNF、mBDNF、组织纤溶酶原激活剂（tPA）、酪氨酸激酶受体B（TrkB）和p75神经营养素受体（p75NTR）的蛋白表达水平：结果：与假手术组相比，神经功能评分、脑梗死体积百分比、海马中proBDNF和p75NTR蛋白的表达水平均有所升高（PPPPP结论：EA可缓解学习和记忆障碍：EA能缓解IR大鼠的学习和记忆损伤，这可能与其上调tPA蛋白的表达，促进proBDNF向mBDNF转化，从而改善突触可塑性的功能有关。

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来源期刊

针刺研究 Medicine-Medicine (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture. The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.