Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer.

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-05-02 DOI:10.1080/21691401.2024.2347369

Hanieh Montaseri, Heidi Abrahamse

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Abstract

Spherical gold/polyacrylic acid (Au/PAA) polymer-inorganic Janus nanoparticles (JNPs) with simultaneous therapeutic and targeting functions were fabricated. The obtained Au/PAA JNPs were further selectively functionalized with folic acid (FA) and thiol PEG amine (SH-PEG-NH₂) on Au sides to provide superior biocompatibility and active targeting, while the other PAA sides were loaded with 5-aminolevulinic acid (5-ALA) to serve as a photosensitizer (PS) for photodynamic therapeutic (PDT) effects on MCF-7 cancer cells. The PS loading of 5-ALA was found to be 83% with an average hydrodynamic size and z-potential of 146 ± 0.8 nm and -6.40 mV respectively for FA-Au/PAA-ALA JNPs. The in vitro PDT study of the JNPs on MCF-7 breast cancer cells under 636 nm laser irradiation indicated the cell viability of 24.7% ± 0.5 for FA-Au/PAA-ALA JNPs at the IC50 value of 0.125 mM. In this regard, the actively targeted FA-Au/PAA-ALA JNPs treatment holds great potential for tumour therapy with high cancer cell-killing efficacy.

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Janus 纳米粒子对乳腺癌的靶向光动力治疗技术。

制备了同时具有治疗和靶向功能的球形金/聚丙烯酸（Au/PAA）聚合物无机 Janus 纳米粒子（JNPs）。所获得的 Au/PAA JNPs 在 Au 面上进一步选择性地添加了叶酸（FA）和硫醇 PEG 氨基（SH-PEG-NH2），以提供优异的生物相容性和活性靶向性；而在 PAA 的另一面则添加了 5-aminolevulinic acid（5-ALA），作为光敏剂（PS），对 MCF-7 癌细胞产生光动力治疗（PDT）效应。研究发现，FA-Au/PAA-ALA JNPs 的 5-ALA PS 负载率为 83%，平均流体力学尺寸和 Z 电位分别为 146 ± 0.8 nm 和 -6.40 mV。在 636 nm 激光照射下对 MCF-7 乳腺癌细胞进行的体外光导放疗研究表明，在 IC50 值为 0.125 mM 时，FA-Au/PAA-ALA JNPs 的细胞存活率为 24.7% ± 0.5。由此可见，FA-Au/PAA-ALA JNPs 的主动靶向治疗在肿瘤治疗方面具有巨大潜力，对癌细胞具有很高的杀伤效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.