In Vivo Anti-Hepatocellular Carcinoma Effects of the Chloroform Root Extract of Clausena excavata Burm.

IF 3.3 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Peter Waziri, Richard Auta, Mustapha U Imam, Ben A Chindo, Zakari Ladan, Zainab Mohammed, Samson Wayah, Ja'afar Mohammed, Mohammed I Tahir, Abdurrahman E Ahmad, Yusuf Alhassan, Daniel Tyoapine, Abel S Agbaji
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引用次数: 0

Abstract

Liver cancer is the most common cancer among males in Africa. The disease has a poor prognosis and its treatment is associated with toxicity and resistance. For this reason, numerous herbal combinations are being subjected to anticancer screening to circumvent the shortcomings of the conventional anticancer drugs. In the current study, the in vivo anti-cancer effects of the chloroform root extract of the herb, Clausena excavata Burm were investigated. Liver cancer was induced in mice by a single intraperitoneal injection of diethylnitrosamine (DEN) followed by oral administration of the promoter of carcinogenesis, 2-aminoacetyl fluorine that was mixed with the mice feed. The cytotoxicity of the root extract of C. excavata on liver cancer cells was investigated using liver enzyme, histology, DNA fragmentation and caspases assays. Real time qPCR was conducted to evaluate the effect of the extract on apoptotic genes. The findings revealed that the extract of C. excavata significantly decreased the progression of hepatocarcinogenesis and the toxicity-induced production of the liver enzymes, alanine and aspartate aminotransferases. The histological analyses of the liver tissues revealed evidence of apoptotic cell death. The extract also provoked significant (p < .05) expressions of caspase 9 protein and gene as well as other apoptotic genes (P53, P27, Apaf-1, cytochrome C, bax and bid). Therefore, we postulate that the chloroform root extract of C. excavata induces apoptosis of liver cancer in mice.

Clausena excavata Burm 的氯仿根提取物在体内抗肝细胞癌的作用
肝癌是非洲男性最常见的癌症。这种疾病的预后很差,治疗过程中会产生毒性和耐药性。因此,许多草药组合正在接受抗癌筛选,以规避传统抗癌药物的缺陷。在本研究中,研究人员调查了氯仿根提取物(Clausena excavata Burm)的体内抗癌效果。小鼠通过腹腔注射二乙基亚硝胺(DEN)诱发肝癌,然后口服致癌促进剂--混在小鼠饲料中的 2-氨基乙酰氟。使用肝酶、组织学、DNA 断裂和 caspases 检测法研究了 C. excavata 根提取物对肝癌细胞的细胞毒性。还进行了实时 qPCR 分析,以评估提取物对凋亡基因的影响。研究结果表明,挖苦草提取物能明显降低肝癌发生的进程,以及毒性诱导的肝酶--丙氨酸和天门冬氨酸转氨酶的生成。肝脏组织的组织学分析显示了细胞凋亡的证据。该提取物还能明显诱导小鼠肝癌细胞凋亡。
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来源期刊
Journal of Evidence-based Integrative Medicine
Journal of Evidence-based Integrative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
5.90
自引率
0.00%
发文量
43
审稿时长
15 weeks
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