Spectrofluorometric determination of calcium in the human nasal secretions investigating the association with olfactory function

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-04-26 DOI:10.1016/j.ab.2024.115549

Ahmed H. Abdelazim , Saleh l. Alaqel , Atiah H. Almalki , Adnan Alharbi , Majed A. Algarni , Maram H. Abduljabbar , Mohamed H. Abdelazim

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Abstract

Ionic microenvironment of the nasal secretions especially calcium ions play essential role in the olfactory transmission. However, there is a critical need to determine the free calcium levels in healthy people's nasal secretions in contrast to those of patients with olfactory impairment. A selective spectrofluorometric method was created to quantify nasal calcium levels utilizing its quenching ability to the fluorescence of the functionalized carbon quantum dots. The surface of carbon quantum dots was functionalized with calcium ionophore A23187 and ion association complex, calcium phosphotungstate, to improve the selectively to quantify calcium ions. The functionalized carbon quantum dots exhibited a concentration-dependent fluorescence quenching upon interaction with calcium ions. Different factors influencing the quenching process were done to provide efficient analytical process. The new method, demonstrated accurate calcium determination over the concentration range of 200–4000 ng/mL. The suggested technique was used to measure the calcium in the nasal secretions of both healthy people and patients with olfactory impairment. The findings revealed significantly higher calcium levels in the patient with olfactory dysfunction (healthy vs. patient; 735 ± 20 ng/mL vs. 2987 ± 37 ng/mL, p < 0.05).

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光谱荧光测定法测定人体鼻腔分泌物中的钙，研究其与嗅觉功能的关系。

鼻腔分泌物中的离子微环境，尤其是钙离子在嗅觉传导中起着至关重要的作用。然而，与嗅觉障碍患者相比，健康人鼻腔分泌物中的游离钙水平亟待确定。利用功能化碳量子点对荧光的淬灭能力，创建了一种选择性光谱荧光测定法来量化鼻腔中的钙含量。为了提高钙离子定量的选择性，在碳量子点表面添加了钙离子载体 A23187 和离子结合复合物磷钨酸钙。功能化的碳量子点在与钙离子相互作用时表现出浓度依赖性荧光淬灭。为了提供高效的分析过程，对影响淬灭过程的不同因素进行了研究。新方法可在 200-4000 纳克/毫升的浓度范围内准确测定钙离子。所建议的技术被用于测量健康人和嗅觉障碍患者鼻腔分泌物中的钙。结果显示，嗅觉障碍患者的钙含量明显更高（健康人与患者；735±20 ng/mL 与 2987±37 ng/mL, p

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.