First detection of triazole-resistant aspergillus fumigatus harbouring the TR34/L98H Cyp51A mutation in Burkina Faso.

IF 4.1 2区医学 Q1 DERMATOLOGY

Mycoses Pub Date : 2024-05-01 DOI:10.1111/myc.13732

Isidore W Yerbanga, Katrien Lagrou, Rita Merckx, Seydou Nakanabo Diallo, Jean-Pierre Gangneux, Aymeric Delabarre, Olivier Denis, Hector Rodriguez-Villalobos, Isabel Montesinos, Sanata Bamba

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引用次数: 0

Abstract

Background: Triazole-resistant Aspergillus fumigatus (TRAF) isolates are a growing public health problem with worldwide distribution. Epidemiological data on TRAF is limited in Africa, particularly in West Africa.

Objectives: This study aimed to screen for the environmental presence of TRAF isolates in the indoor air of two hospitals in Burkina Faso.

Materials and methods: Air samples were collected in wards housing patients at risk for invasive aspergillosis, namely infectious diseases ward, internal medicine ward, nephrology ward, pulmonology ward, medical emergency ward and paediatric ward. Sabouraud Dextrose Agar supplemented with triazoles was used to screen the suspected TRAF isolates and EUCAST method to confirm the resistance of suspected isolates. Sequencing of cyp51A gene was used to identify the resistance mechanism of confirmed TRAF isolates.

Results: Of the 198 samples collected and analysed, 67 showed growth of A. fumigatus isolates. The prevalence of TRAF isolates was 3.23% (4/124). One TRAF isolate exhibited a pan-triazole resistance. Sequencing of cyp51A gene identified the TR34/L98H mutation for this pan-triazole resistant isolate. This study showed for the first time the circulation of the pan-azole resistant isolate harbouring the TR34/L98H mutation in Burkina Faso.

Conclusions: These findings emphasise the need to map these TRAF isolates in all parts of Burkina Faso and to establish local and national continuous surveillance of environmental and clinical TRAF isolates in this country.

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在布基纳法索首次发现携带 TR34/L98H Cyp51A 突变的耐三唑曲霉。

背景：耐三唑曲霉菌（TRAF）分离株是一个日益严重的公共卫生问题，其分布范围遍及全球。在非洲，尤其是西非，有关 TRAF 的流行病学数据十分有限：本研究旨在筛查布基纳法索两家医院室内空气中 TRAF 分离物的环境存在情况：在传染病房、内科病房、肾科病房、肺科病房、急诊病房和儿科病房等有侵袭性曲霉病风险的病人所在的病房收集空气样本。使用添加三唑类药物的沙保露葡萄糖琼脂筛选疑似 TRAF 分离物，并使用 EUCAST 方法确认疑似分离物的耐药性。对 cyp51A 基因进行测序，以确定经证实的 TRAF 分离物的抗药性机制：结果：在收集和分析的 198 份样本中，有 67 份显示有烟曲霉分离株生长。TRAF 分离物的流行率为 3.23%（4/124）。一个 TRAF 分离物表现出泛三唑抗性。对 cyp51A 基因进行测序后发现，该抗泛三唑分离物存在 TR34/L98H 突变。这项研究首次显示了携带 TR34/L98H 突变的泛唑抗性分离物在布基纳法索的流行情况：这些发现强调了在布基纳法索所有地区绘制这些 TRAF 分离物地图的必要性，以及在该国建立地方和全国性的环境和临床 TRAF 分离物持续监测的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycoses 医学-皮肤病学

CiteScore

10.00

自引率

8.20%

发文量

143

审稿时长

6-12 weeks

期刊介绍： The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans as well as on the biology of pathogenic fungi. Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. The journal Mycoses is therefore of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians and clinicians interested in fungal infections.