Antibacterial potential of Euphorbia canariensis against Pseudomonas aeruginosa bacteria causing respiratory tract infections.

IF 4.5 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Badriyah Alotaibi, Engy Elekhnawy, Thanaa A El-Masry, Asmaa Saleh, Manal E Alosaimi, Khalid Nijr Alotaibi, Walaa A Negm
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引用次数: 0

Abstract

The widespread dissemination of bacterial resistance has led to great attention being paid to finding substitutes for traditionally used antibiotics. Plants are rich in various phytochemicals that could be used as antibacterial therapies. Here, we elucidate the phytochemical profile of Euphorbia canariensis ethanol extract (EMEE) and then elucidate the antibacterial potential of ECEE against Pseudomonas aeruginosa clinical isolates. ECEE showed minimum inhibitory concentrations ranging from 128 to 512 µg/mL. The impact of ECEE on the biofilm-forming ability of the tested isolates was elucidated using crystal violet assay and qRT-PCR to study its effect on the gene expression level. ECEE exhibited antibiofilm potential, which resulted in a downregulation of the expression of the biofilm genes (algD, pelF, and pslD) in 39.13% of the tested isolates. The antibacterial potential of ECEE was studied in vivo using a lung infection model in mice. A remarkable improvement was observed in the ECEE-treated group, as revealed by the histological and immunohistochemical studies. Also, ELISA showed a noticeable decrease in the oxidative stress markers (nitric oxide and malondialdehyde). The gene expression of the proinflammatory marker (interleukin-6) was downregulated, while the anti-inflammatory biomarker was upregulated (interleukin-10). Thus, clinical trials should be performed soon to explore the potential antibacterial activity of ECEE, which could help in our battle against resistant pathogenic bacteria.

加那利大戟对引起呼吸道感染的铜绿假单胞菌的抗菌潜力。
由于细菌抗药性的广泛传播,人们开始高度重视寻找传统抗生素的替代品。植物富含各种植物化学物质,可用作抗菌疗法。在此,我们对大戟科植物乙醇提取物(EMEE)的植物化学成分进行了分析,然后阐明了ECEE对铜绿假单胞菌临床分离株的抗菌潜力。ECEE 的最小抑菌浓度为 128 至 512 µg/mL。利用水晶紫测定法和 qRT-PCR 研究了 ECEE 对基因表达水平的影响,从而阐明了 ECEE 对受试分离菌生物膜形成能力的影响。结果表明,ECEE 具有抗生物膜的潜能,可导致 39.13% 的受试分离物的生物膜基因(algD、pelF 和 pslD)表达下调。研究人员利用小鼠肺部感染模型对环己基氨基甲酸乙酯的抗菌潜力进行了体内研究。组织学和免疫组化研究表明,经 ECEE 处理的小鼠肺部感染情况明显好转。此外,酶联免疫吸附试验(ELISA)显示,氧化应激标记物(一氧化氮和丙二醛)明显减少。促炎标志物(白细胞介素-6)的基因表达下调,而抗炎生物标志物(白细胞介素-10)则上调。因此,应尽快开展临床试验,探索环己基氨基甲酸乙酯的潜在抗菌活性,这将有助于我们与具有抗药性的病原菌作斗争。
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来源期刊
Artificial Cells, Nanomedicine, and Biotechnology
Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
10.90
自引率
0.00%
发文量
48
审稿时长
20 weeks
期刊介绍: Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.
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