Development of Simultaneous HPTLC Method and Validation for the Quality Assessment of Ayurvedic Formulation-Ayush Kvatha Churna by Using Marker Compound Rosmarinic Acid, Trans-Cinnamaldehyde and Piperine.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-05 DOI:10.1093/chromsci/bmae019

Umakant Sahu, Nagendra Singh Chauhan, Arun Kumar Singh Parihar, Kamleshwar Singh Karbhal, Shrikant R Inchulkar, Prashant Kumar Gupta, Rajesh Kumar Singh

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引用次数: 0

Abstract

Ayurveda emphasizes the propagation of nature in maintaining health. In the present scenario, we have seen the faith of people in herbal drugs during the Covid 19 outbreak. The raises in the number of peoples have been using herbal drugs to boost immunity against infectious diseases shows the popularity of this ancient system of medicine. The standardization of Ayush Kvatha Churna (AKC), work set out to establish a straightforward, accurate and sensitive HPTLC method for the identification and quantification of marker compounds. The Rosmarinic acid, trans-Cinnamaldehyde and Piperine were used for the estimation of markers in Ayush Kvatha Churna by using HPTLC with a solvent system, consisting of Toluene: Ethyl acetate: Ethyl alcohol: Formic acid (5.6:2.4:2: 0.3 v/v/v/v). The Rf value 0.33 for Rosmarinic Acid, 0.69 for Piperine and 0.77 for trans-Cinnamaldehyde was observed and it is exactly complying with the corresponding bands in Ayush Kvatha Churna. The technique has been effectively verified and validated, enabling it to be used for the standardization or quantitative analysis of Rosmarinic acid, trans-Cinnamaldehyde and piperine in Ayush Kvatha Churna.

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利用标记化合物香豆素酸、反式肉桂醛和胡椒碱，为阿育吠陀配方--Ayush Kvatha Churna 的质量评估开发同步 HPTLC 方法并进行验证。

阿育吠陀强调顺应自然，维护健康。在当前形势下，我们看到了人们在科维德 19 爆发期间对草药的信任。越来越多的人使用草药来提高免疫力，以抵御传染病，这表明这一古老的医学体系广受欢迎。为了实现阿育王草药（AKC）的标准化，研究人员着手建立一种简单、准确和灵敏的 HPTLC 方法，用于识别和定量标记化合物。乙酸乙酯乙醇甲酸（5.6:2.4:2: 0.3 v/v/v/v）。观察到玫瑰果酸的 Rf 值为 0.33，胡椒碱的 Rf 值为 0.69，反式肉桂醛的 Rf 值为 0.77，与 Ayush Kvatha Churna 中的相应条带完全一致。该技术得到了有效的验证和确认，可用于对 Ayush Kvatha Churna 中的迷迭香酸、反式肉桂醛和胡椒碱进行标准化或定量分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.