Twin Study: The Factors Affecting the Serum LDL-C and HDL-C Levels and an RNA-Seq Analysis in Mononuclear Cells in Monozygotic Twins.

IF 3 2区 医学 Q2 PERIPHERAL VASCULAR DISEASE
Journal of atherosclerosis and thrombosis Pub Date : 2024-11-01 Epub Date: 2024-04-27 DOI:10.5551/jat.64882
Sae Nishihara, Masahiro Koseki, Katsunao Tanaka, Takashi Omatsu, Hiroshi Sawabe, Hiroyasu Inui, Ayami Saga, Takeshi Okada, Tomoaki Higo, Tohru Ohama, Makoto Nishida, Yasushi Sakata, Mikio Watanabe
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引用次数: 0

Abstract

Aim: A twin study is a valuable tool for elucidating the acquired factors against lifestyle diseases such as dyslipidemia, diabetes mellitus, and obesity. We aimed 1. to investigate the factors that affect low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in monozygotic (MZ) twins, and 2. to identify genes which expression levels changed in pairs with large differences in LDL-C or HDL-C levels.

Methods: The registered database at the Center for Twin Research, Osaka University, containing 263 pairs of MZ twins, was analyzed. 1. The effects of smoking, exercise, nutritional factors, and anthropometric and biochemical parameters on LDL-C or HDL-C levels were examined in MZ twins. 2. RNA sequencing in the peripheral blood mononuclear cells of 59 pairs was analyzed for large differences of LDL-C or HDL-C groups.

Results: 1. The ΔLDL-C levels were significantly associated with an older age, the ΔTG levels, and ΔBMI. ΔHDL-C levels were associated with the ΔBMI, ΔTG, ΔTP, and ΔLDL-C levels. The HDL-C levels were affected by smoking and exercise habits. The intakes of cholesterol and saturated fatty acids were not associated with the LDL-C or HDL-C levels. 2. An RNA sequencing analysis revealed that the expression of genes related to the TLR4 and IFNG pathways was suppressed in accordance with the HDL-C levels in the larger ΔHDL-C group among the 59 pairs.

Conclusion: We identified the factors affecting the LDL-C or HDL-C levels in monozygotic twins. In addition, some types of inflammatory gene expression in peripheral blood mononuclear cells were suppressed in accordance with the HDL-C levels, thus suggesting the importance of weight management and exercise habits in addition to dietary instructions to control the LDL-C or HDL-C levels.

双胞胎研究:影响血清低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平的因素以及单卵双生子单核细胞中的 RNA 序列分析。
目的:双生子研究是阐明血脂异常、糖尿病和肥胖等生活方式疾病的后天因素的重要工具。我们的目的是:1.研究影响单卵双生子(MZ)低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)的因素;2.确定在 LDL-C 或 HDL-C 水平差异较大的配对中表达水平发生变化的基因:方法:分析大阪大学双胞胎研究中心的注册数据库,其中包含 263 对 MZ 双胞胎。1.1. 研究了吸烟、运动、营养因素以及人体测量和生化指标对 MZ 双胞胎低密度脂蛋白胆固醇或高密度脂蛋白胆固醇水平的影响。2.2. 对 59 对双胞胎的外周血单核细胞进行 RNA 测序,分析低密度脂蛋白胆固醇(LDL-C)或高密度脂蛋白胆固醇(HDL-C)组间的巨大差异:1.ΔLDL-C水平与年龄、ΔTG水平和ΔBMI显著相关。ΔHDL-C水平与ΔBMI、ΔTG、ΔTP和ΔLDL-C水平相关。高密度脂蛋白胆固醇水平受吸烟和运动习惯的影响。胆固醇和饱和脂肪酸的摄入量与低密度脂蛋白胆固醇或高密度脂蛋白胆固醇水平无关。2.2. RNA 测序分析表明,在 59 对ΔHDL-C 较大的组别中,与 TLR4 和 IFNG 通路相关的基因表达随 HDL-C 水平的升高而受到抑制:结论:我们发现了影响单卵双生子低密度脂蛋白胆固醇(LDL-C)或高密度脂蛋白胆固醇(HDL-C)水平的因素。结论:我们发现了影响单卵双生子低密度脂蛋白胆固醇(LDL-C)或高密度脂蛋白胆固醇(HDL-C)水平的因素,此外,外周血单核细胞中某些类型的炎症基因表达随着高密度脂蛋白胆固醇(HDL-C)水平的升高而受到抑制,这表明除了饮食指导外,体重管理和运动习惯对控制低密度脂蛋白胆固醇(LDL-C)或高密度脂蛋白胆固醇(HDL-C)水平也很重要。
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来源期刊
CiteScore
6.60
自引率
15.90%
发文量
271
审稿时长
1 months
期刊介绍: JAT publishes articles focused on all aspects of research on atherosclerosis, vascular biology, thrombosis, lipid and metabolism.
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