Assessment of total aflatoxin and ochratoxin A in poultry feed ingredients by thin-layer chromatography and enzyme-linked immunosorbent assay.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-31 eCollection Date: 2024-03-01 DOI:10.5455/javar.2024.k754

Mustafa Rahim, Nadeem Rashid, Khanoranga, Waqas Ahmad, Zainia Rehmat, Afroz Rais, Zainab Siddique, Kashif Kamran

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引用次数: 0

Abstract

Objective: This study was conducted to evaluate the prevalence of total aflatoxin (AF) and ochratoxin A (OTA) in poultry feed ingredients under different environmental conditions during the summer and winter seasons, while the hygiene quality of the feed ingredient was assessed through viable fungal count (VFC).

Materials and methods: A total of 288 poultry feed ingredients (n = 96 each) samples were collected from different poultry shops, which were initially analyzed for the presence of AF and OTA through thin layer chromatography (TLC) and then confirmed the contamination concentration through the enzyme-linked immunosorbent assay method.

Results: The results of the current study confirmed the incidence of contamination with AF and OTA by TLC and ELISA methods. The contamination level of AF ranged from 26.09 to 50.56 (mean = 41.22 ± 9.45) μg/kg, whereas the contamination level of OTA ranged from 50.13 to 6.21 (mean 42.60 ± 6.21) μg/kg. The contamination level of AF was found to be above the permissible level set by the Food and Drug Administration (20 μg/kg), whereas the contamination level of OTA was below the permissible limits. Moreover, the VFC values were also below the recommended level. The results showed that the association between AF, OTA, and moisture content was significant (p < 0.05).

Conclusion: Mycotoxin contamination was significantly (p < 0.05) highest in the winter season. These findings suggested that continuous monitoring regimes might prevent mycotoxin contamination in poultry feed ingredients.

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利用薄层色谱法和酶联免疫吸附测定法评估家禽饲料配料中的黄曲霉毒素和赭曲霉毒素 A 总含量。

研究目的本研究旨在评估在夏季和冬季不同环境条件下，家禽饲料成分中总黄曲霉毒素（ AF ）和赭曲霉毒素 A （ OTA ）的含量：从不同的家禽店收集了共 288 份家禽饲料原料样本（每份样本 n = 96），通过薄层色谱法（TLC）初步分析其是否含有 AF 和 OTA，然后通过酶联免疫吸附测定法确认其污染浓度：结果：本次研究的结果证实，通过薄层色谱法和酶联免疫吸附测定法，AF 和 OTA 的污染发生率很高。AF 的污染水平在 26.09 至 50.56（平均值 = 41.22 ± 9.45）微克/千克之间，而 OTA 的污染水平在 50.13 至 6.21（平均值 42.60 ± 6.21）微克/千克之间。发现 AF 的污染水平高于食品和药物管理局规定的允许水平（20 微克/千克），而 OTA 的污染水平则低于允许限值。此外，VFC 值也低于建议水平。结果表明，AF、OTA 和水分含量之间存在显著关联（p < 0.05）：结论：霉菌毒素污染在冬季明显最高（p < 0.05）。这些结果表明，持续的监测制度可以防止家禽饲料原料中霉菌毒素的污染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.