Cell-free supernatant of Lactococcus lactis IDCC 2301 exerts anti-inflammatory effects in LPS-induced macrophages via NF-κB and MAPK signaling pathways.

IF 3 4区医学 Q2 MICROBIOLOGY

Beneficial microbes Pub Date : 2024-04-30 DOI:10.1163/18762891-bja00008

M Lee, S A Chae, Y J Lee, H J Jeon, S-C Hong, M Shin, Y H Jung, J Yang

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引用次数: 0

Abstract

This study investigated the anti-inflammatory effects of cell-free supernatant of Lactococcus lactis IDCC 2301 on lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Expression of inflammatory mediators and cytokines, and the production of nitric oxide (NO) and prostaglandin E2 (PGE2) were qualitatively analysed. The expression of signal transductors in inflammatory cascades was quantified by western blot. Treatment with cell-free supernatant of L. lactis IDCC 2301 significantly decreased the mRNA expression levels of tumour necrosis factor (TNF-α) and interleukins including IL-1β and IL-6. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) were also remarkably reduced in LPS-induced macrophages after the treatment. Furthermore, L. lactis IDCC 2301 reduced the levels of both dephosphorylated and phosphorylated forms of nuclear factor-kappa B (NF-κB), IκB-α, extracellular signal-regulated kinases (ERK), c-Jun amino-terminal kinases (JNK), and p38 in LPS-induced RAW 264.7 cells. Therefore, L. lactis IDCC 2301 shows anti-inflammatory activity by suppressing the NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways.

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乳球菌 IDCC 2301 的无细胞上清液通过 NF-κB 和 MAPK 信号通路对 LPS 诱导的巨噬细胞产生抗炎作用。

本研究探讨了乳球菌 IDCC 2301 的无细胞上清液对脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞的抗炎作用。定性分析了炎症介质和细胞因子的表达以及一氧化氮（NO）和前列腺素 E2（PGE2）的产生。炎症级联中信号转导物的表达则通过 Western 印迹进行量化。用乳酸菌 IDCC 2301 的无细胞上清液处理后，肿瘤坏死因子（TNF-α）和白细胞介素（包括 IL-1β 和 IL-6）的 mRNA 表达水平明显下降。治疗后，LPS 诱导的巨噬细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶（COX-2）的水平也明显降低。此外，乳酸菌 IDCC 2301 还降低了 LPS 诱导的 RAW 264.7 细胞中去磷酸化和磷酸化形式的核因子-卡巴 B（NF-κB）、IκB-α、细胞外信号调节激酶（ERK）、c-Jun 氨基末端激酶（JNK）和 p38 的水平。因此，L. lactis IDCC 2301 通过抑制 NF-κB 和丝裂原活化蛋白激酶（MAPK）信号通路而显示出抗炎活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Beneficial microbes MICROBIOLOGY-NUTRITION & DIETETICS

CiteScore

7.90

自引率

1.90%

发文量

审稿时长

>12 weeks

期刊介绍： Beneficial Microbes is a peer-reviewed scientific journal with a specific area of focus: the promotion of the science of microbes beneficial to the health and wellbeing of man and animal. The journal contains original research papers and critical reviews in all areas dealing with beneficial microbes in both the small and large intestine, together with opinions, a calendar of forthcoming beneficial microbes-related events and book reviews. The journal takes a multidisciplinary approach and focuses on a broad spectrum of issues, including safety aspects of pro- & prebiotics, regulatory aspects, mechanisms of action, health benefits for the host, optimal production processes, screening methods, (meta)genomics, proteomics and metabolomics, host and bacterial physiology, application, and role in health and disease in man and animal. Beneficial Microbes is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as those of policy makers and regulators. The journal will have five major sections: * Food, nutrition and health * Animal nutrition * Processing and application * Regulatory & safety aspects * Medical & health applications In these sections, topics dealt with by Beneficial Microbes include: * Worldwide safety and regulatory issues * Human and animal nutrition and health effects * Latest discoveries in mechanistic studies and screening methods to unravel mode of action * Host physiology related to allergy, inflammation, obesity, etc. * Trends in application of (meta)genomics, proteomics and metabolomics * New developments in how processing optimizes pro- & prebiotics for application * Bacterial physiology related to health benefits