Angiogenic potential in periodontal stem cells from upper and lower jaw: A pilot study

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Hanna Malyaran, Chloé Radermacher, Rogerio B. Craveiro, Mark P. Kühnel, Danny Jonigk, Michael Wolf, Sabine Neuss
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引用次数: 0

Abstract

Background

Teeth and supporting oral tissues are attractive and accessible sources of stem cells. Periodontal ligament stem cells (PDLSC) are readily isolated from extracted third molars, and exhibit the ability to self-renew and differentiate into multiple mesodermal cell fates. Clinical experience suggests that the exact location of periodontal defects affects the oral bone remodeling and wound healing. Compared to the mandible, the maxilla heals quicker and more efficiently. Angiogenesis is key in tissue regeneration including dental tissues, yet few studies focus on the angiogenic potential of PDLSC, none of which considered the differences between upper and lower jaw PDLSC (u-PDLSC and l-PDLSC, respectively).

Methods

Here we studied the angiogenic potential of u-PDLSC and l-PDLSC and compared the results to well-established mesenchymal stem cells (MSC). Cells were characterized in terms of surface markers, proliferation, and vascular endothelial growth factor (VEGF) secretion, and angiogenic assays were performed. Newly formed capillaries were stained with CD31, and their expression of platelet endothelial cell adhesion molecule (PECAM-1), angiopoietin 2 (ANGPT2), and vascular endothelial growth factor receptor 1 and 2 (VEGFR-1, VEGFR-2) were measured.

Results

Periodontal stem cells from the upper jaw showed a higher proliferation capacity, secreted more VEGF, and formed capillary networks faster and denser than l-PDLSC. Gene expression of angiogenesis-related genes was significantly higher in u-PDLSC than in l-PDLSC or MSC, given that culture conditions were suitable.

Conclusion

The oral cavity is a valuable source of stem cells, particularly PDLSC, which are promising for oral tissue engineering due to their robust growth, lifelong accessibility, low immunogenicity, and strong differentiation potential. Notably, u-PDLSC exhibit higher VEGF secretion and accelerate capillary formation compared to l-PDLSC or MSC. This study suggests a potential molecular mechanism in capillary formation, emphasizing the significance of precise location isolation of PDLSC.

Abstract Image

上下颌骨牙周干细胞的血管生成潜力:试点研究
背景牙齿和支持性口腔组织是极具吸引力且容易获取的干细胞来源。牙周韧带干细胞(PDLSC)很容易从拔出的第三磨牙中分离出来,并表现出自我更新和分化为多种中胚层细胞命运的能力。临床经验表明,牙周缺损的确切位置会影响口腔骨重塑和伤口愈合。与下颌骨相比,上颌骨的愈合速度更快,效率更高。血管生成是包括牙科组织在内的组织再生的关键,但很少有研究关注PDLSC的血管生成潜能,也没有研究考虑到上颌和下颌PDLSC(分别为u-PDLSC和l-PDLSC)之间的差异。我们对细胞的表面标记、增殖和血管内皮生长因子(VEGF)分泌进行了表征,并进行了血管生成试验。用CD31对新形成的毛细血管进行染色,并测量其血小板内皮细胞粘附分子(PECAM-1)、血管生成素2(ANGPT2)、血管内皮生长因子受体1和2(VEGFR-1、VEGFR-2)的表达。在培养条件适宜的情况下,u-PDLSC血管生成相关基因的表达明显高于l-PDLSC或间叶干细胞。值得注意的是,与l-PDLSC或间叶干细胞相比,u-PDLSC表现出更高的血管内皮生长因子分泌量,并能加速毛细血管的形成。这项研究提出了毛细血管形成的潜在分子机制,强调了精确定位分离 PDLSC 的重要性。
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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