Mitochondrial administration alleviates lead- and cadmium-induced toxicity in rat renal cells

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Biology International Pub Date : 2024-04-29 DOI:10.1002/cbin.12165

Enayatollah Seydi, Alireza Kanani Nambani, Ali Khorasani, Farzaneh Kamranfar, Abdollah Arjmand, Jalal Pourahmad

{"title":"Mitochondrial administration alleviates lead- and cadmium-induced toxicity in rat renal cells","authors":"Enayatollah Seydi, Alireza Kanani Nambani, Ali Khorasani, Farzaneh Kamranfar, Abdollah Arjmand, Jalal Pourahmad","doi":"10.1002/cbin.12165","DOIUrl":null,"url":null,"abstract":"<p>The role of heavy metals such as lead (Pb) and cadmium (Cd) in the etiology of many diseases has been proven. Also, these heavy metals can affect the normal mitochondrial function. Mitochondrial administration therapy is one of the methods used by researchers to help improve mitochondrial defects and diseases. The use of isolated mitochondria as a therapeutic approach has been investigated in in vivo and in vitro studies. Accordingly, in this study, the effects of mitochondrial administration on the improvement of toxicity caused by Pb and Cd in renal proximal tubular cells (RPTC) have been investigated. The results showed that treatment to Pb and Cd caused an increase in the level of free radicals, lipid peroxidation (LPO) content, mitochondrial and lysosomal membrane damage, and also a decrease in the reduced glutathione content in RPTC. In addition, reports have shown an increase in oxidized glutathione content and changes in energy (ATP) levels. Following, the results have shown the protective role of mitochondrial administration in improving the toxicity caused by Pb and Cd in RPTC. Furthermore, the mitochondrial internalization into RPT cells is mediated through actin-dependent endocytosis. So, it could be suggested that the treatment of Pb- and Cd-induced cytotoxicity in RPTC could be carried out through mitochondria administration.</p>","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":"48 7","pages":"984-996"},"PeriodicalIF":3.3000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Biology International","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cbin.12165","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The role of heavy metals such as lead (Pb) and cadmium (Cd) in the etiology of many diseases has been proven. Also, these heavy metals can affect the normal mitochondrial function. Mitochondrial administration therapy is one of the methods used by researchers to help improve mitochondrial defects and diseases. The use of isolated mitochondria as a therapeutic approach has been investigated in in vivo and in vitro studies. Accordingly, in this study, the effects of mitochondrial administration on the improvement of toxicity caused by Pb and Cd in renal proximal tubular cells (RPTC) have been investigated. The results showed that treatment to Pb and Cd caused an increase in the level of free radicals, lipid peroxidation (LPO) content, mitochondrial and lysosomal membrane damage, and also a decrease in the reduced glutathione content in RPTC. In addition, reports have shown an increase in oxidized glutathione content and changes in energy (ATP) levels. Following, the results have shown the protective role of mitochondrial administration in improving the toxicity caused by Pb and Cd in RPTC. Furthermore, the mitochondrial internalization into RPT cells is mediated through actin-dependent endocytosis. So, it could be suggested that the treatment of Pb- and Cd-induced cytotoxicity in RPTC could be carried out through mitochondria administration.

查看原文本刊更多论文

线粒体给药可减轻铅和镉诱导的大鼠肾细胞毒性

铅（Pb）和镉（Cd）等重金属在许多疾病的病因中的作用已得到证实。此外，这些重金属还会影响线粒体的正常功能。线粒体管理疗法是研究人员用来帮助改善线粒体缺陷和疾病的方法之一。已在体内和体外研究中对使用分离线粒体作为治疗方法进行了调查。因此，本研究调查了线粒体给药对改善肾近曲小管细胞（RPTC）中铅和镉引起的毒性的影响。结果表明，铅和镉处理导致 RPTC 自由基水平、脂质过氧化（LPO）含量、线粒体和溶酶体膜损伤增加，还导致还原型谷胱甘肽含量降低。此外，有报告显示氧化谷胱甘肽含量增加，能量（ATP）水平发生变化。研究结果表明，线粒体管理在改善 RPTC 中铅和镉的毒性方面起着保护作用。此外，线粒体在 RPT 细胞中的内化是通过肌动蛋白依赖性内吞作用介导的。因此，可以认为可以通过线粒体给药来治疗铅和镉诱导的 RPTC 细胞毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cell Biology International 生物-细胞生物学

CiteScore

7.60

自引率

0.00%

发文量

208

审稿时长

1 months

期刊介绍： Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.