Changes in nutrient availability substantially alter bacteria and extracellular enzymatic activities in Antarctic soils

Abstract

In polar regions, global warming has accelerated the melting of glacial and buried ice, resulting meltwater run-off and mobilisation of surface nutrients. Yet, the short-term effects of altered nutrient regimes, on the diversity and function of soil microbiota in poly-extreme environments such as Antarctica, remains poorly understood. We studied these effects by simulating such environments via constructing soil microcosms through augmented carbon, nitrogen, and moisture supplements. Addition of nitrogen significantly decreased the diversity of Antarctic soil microbial assemblages, compared with other treatment groups. Other treatments led to shift in relative abundances of these microbial assemblages with random distributional patterns. Only nitrogen treatment appeared to show clear community structural patterns, with increase in abundance of Proteobacteria (Gammaproteobateria) and decrease in Verrucomicrobiota (Chlamydiae, Verrucomicrobiae). Effects of extracellular enzyme activities and soil parameters on changes in microbial taxa also showed significance impacts of nitrogen treatment. Microbial response to nutrient addition was predicted using structural equation modelling which revealed that nutrient source and extracellular enzyme activities were positive predictors of microbial diversity. Our study highlights the effect of nitrogen addition on Antarctic soil microorganisms which showed resilience to nutrient increases. Rather than being resistant to change, these microorganisms rapidly responded to augmented nutrient regimes.