Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2024-05-01 DOI:10.1111/jop.13541

Farah Essgui Orellana Martinez, Thâmara Manoela Marinho Bezerra, Ana Paula Negreiros Nunes Alves, Isabelle Joyce Lima Silva Fernandes, Fabricio Bitu Sousa, Paulo Goberlânio de Barros Silva, Mário Rogério Lima Mota

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引用次数: 0

Abstract

Background

To evaluate the presence of myofibroblasts (MFs) in the development of lip carcinogenesis, through the correlation of clinical, histomorphometric and immunohistochemical parameters, in actinic cheilitis (ACs) and lower lip squamous cell carcinomas (LLSCCs).

Methods

Samples of ACs, LLSCCs, and control group (CG) were prepared by tissue microarray (TMA) for immunohistochemical TGF-β, α-SMA, and Ki-67 and histochemical hematoxylin and eosin, picrosirius red, and verhoeff van gieson reactions. Clinical and microscopic data were associated using the Mann–Whitney, Kruskal-Wallis/Dunn, and Spearman correlation tests (SPSS, p < 0.05).

Results

ACs showed higher number of α-SMA⁺ MFs when compared to CG (p = 0.034), and these cells were associated with the vertical expansion of solar elastosis (SE) itself (p = 0.027). Areas of SE had lower deposits of collagen (p < 0.001), immunostaining for TGF-β (p < 0.001), and higher density of elastic fibers (p < 0.05) when compared to areas without SE. A positive correlation was observed between high-risk epithelial dysplasia (ED) and the proximity of SE to the dysplastic epithelium (p = 0.027). LLSCCs showed a higher number of α-SMA⁺ MFs about CG (p = 0.034), as well as a reduction in the deposition of total collagen (p = 0.009) in relation to ACs and CG. There was also a negative correlation between the amount of α-SMA⁺ cells and the accumulation of total collagen (p = 0.041). Collagen and elastic density loss was higher in larger tumors (p = 0.045) with nodal invasion (p = 0.047).

Conclusions

Our findings show the possible role of MFs, collagen fibers, and elastosis areas in the lip carcinogenesis process.

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评估肌成纤维细胞和细胞外基质结构成分与口角炎和下唇鳞状细胞癌组织病理学参数的关系

背景通过临床、组织形态学和免疫组化参数的相关性，评估肌成纤维细胞（MFs）在动胬肉（ACs）和下唇鳞状细胞癌（LLSCCs）唇癌发生过程中的存在情况。方法用组织芯片（TMA）制备下唇鳞状细胞癌（ACs）、下唇鳞状细胞癌（LLSCCs）和对照组（CG）的样本，进行免疫组化 TGF-β、α-SMA 和 Ki-67，以及组织化学苏木精和伊红、苦橙红和verhoeff van gieson 反应。采用 Mann-Whitney、Kruskal-Wallis/Dunn 和 Spearman 相关性检验（SPSS，p <0.05）对临床和显微镜数据进行关联检验。与没有出现日光性弹力纤维症的区域相比，出现日光性弹力纤维症的区域胶原蛋白沉积较低（p = 0.001），TGF-β免疫染色较低（p = 0.001），弹力纤维密度较高（p = 0.05）。在高风险上皮发育不良（ED）与SE与发育不良上皮的邻近程度之间观察到正相关（p = 0.027）。与 AC 和 CG 相比，LLSCC 显示出更多的α-SMA+ MFs（p = 0.034），以及总胶原沉积的减少（p = 0.009）。α-SMA+细胞的数量与总胶原蛋白的积累之间也呈负相关（p = 0.041）。结论我们的研究结果表明，中频、胶原纤维和弹性区域在唇癌发生过程中可能起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.