Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-05 DOI:10.1093/chromsci/bmae018

Saeedeh Jafari, Abdollah Hematian Sourki

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Abstract

A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.

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甘蔗渣提取物中葡萄糖和木糖的高效液相色谱法的开发与验证

为鉴定和定量水解甘蔗渣提取物中的葡萄糖和木糖，开发了一种有效的快速高效液相色谱-折光率（RI）分析方法。采用 Eurokat® H 色谱柱（300 × 8 mm, 10 μm），以 0.01 N 硫酸溶液为流动相，流速为 0.6 mL/min，检测温度为 75°C。该方法的准确度、精密度、线性、稳健性、不确定性、检出限（LOD）和定量限（LOQ）均得到了验证。每个样品的色谱分析总时间约为 11 分钟。葡萄糖和木糖在 11-100 μg/100 μL 的浓度范围内线性关系良好。LOD 为 0.8 ppm，LOQ 为 2.5 ppm。高回收率和低相对标准偏差证实了该方法适用于蔗渣提取物中葡萄糖和木糖的测定。所提出的 HPLC-RI 方法准确、快速、稳健，由于分析物的保留时间较短，所需的运行时间也较短，而且由于柱温较低，能耗也较低。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.