Priyanshu Shukla, Ashis Kumar Bera, Sriya Yeleswarapu, Falguni Pati
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引用次数: 0
Abstract
3D bioprinting allows rapid automated fabrication and can be applied for high throughput generation of biomimetic constructs for in vitro drug screening. Decellularized extracellular matrix (dECM) hydrogel is a popular biomaterial choice for tissue engineering and studying carcinogenesis as a tumor microenvironmental mimetic. This study proposes a method for high throughput bioprinting with decellularized adipose tissue (DAT) based hydrogels for 3D breast cancer modeling. A comparative analysis of decellularization protocol using detergent-based and detergent-free decellularization methods for caprine-origin adipose tissue is performed, and the efficacy of dECM hydrogel for 3D cancer modeling is assessed. Histological, biochemical, morphological, and biological characterization and analysis showcase the cytocompatibility of DAT hydrogel. The rheological property of DAT hydrogel and printing process optimization is assessed to select a bioprinting window to attain 3D breast cancer models. The bioprinted tissues are characterized for cellular viability and tumor cell-matrix interactions. Additionally, an approach for breast cancer modeling is shown by performing rapid high throughput bioprinting in a 96-well plate format, and in vitro drug screening using 5-fluorouracil is performed on 3D bioprinted microtumors. The results of this study suggest that high throughput bioprinting of cancer models can potentially have downstream clinical applications like multi-drug screening platforms and personalized disease models.
三维生物打印技术可实现快速自动化制造,并可用于高通量生成体外药物筛选所需的仿生物构建体。脱细胞细胞外基质(dECM)水凝胶作为一种肿瘤微环境模拟物,是组织工程和致癌研究的常用生物材料。本研究提出了一种基于脱细胞脂肪组织(DAT)水凝胶的高通量生物打印方法,用于三维乳腺癌建模。该研究比较分析了基于去垢剂和不含去垢剂的毛细血管源脂肪组织脱细胞方案,并评估了用于三维癌症建模的 dECM 水凝胶的功效。组织学、生物化学、形态学和生物学表征与分析显示了 DAT 水凝胶的细胞相容性。通过评估 DAT 水凝胶的流变特性和打印工艺优化,选择生物打印窗口,实现三维乳腺癌模型。对生物打印组织的细胞活力和肿瘤细胞与基质的相互作用进行了表征。此外,通过在 96 孔板格式中进行快速高通量生物打印,展示了一种乳腺癌建模方法,并在三维生物打印微瘤上使用 5-氟尿嘧啶进行了体外药物筛选。这项研究的结果表明,高通量生物打印癌症模型有可能在下游临床中得到应用,如多药筛选平台和个性化疾病模型。
期刊介绍:
Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals.
Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers.
With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.