Reference intervals for bone marrow cells in juvenile and young adult cats bone marrow cytology

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2024-04-29 DOI:10.1111/vcp.13349

Nilson J. S. Nunes, Stella de Faria Valle, Felipe Y. Okano, Bruno A. Almeida, Angélica Menin, Fernanda V. A. Costa, Naila C. B. Duda, Félix H. D. González

{"title":"Reference intervals for bone marrow cells in juvenile and young adult cats bone marrow cytology","authors":"Nilson J. S. Nunes, Stella de Faria Valle, Felipe Y. Okano, Bruno A. Almeida, Angélica Menin, Fernanda V. A. Costa, Naila C. B. Duda, Félix H. D. González","doi":"10.1111/vcp.13349","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The current bone marrow (BM) reference intervals (RI) are based on a limited number of cats. Age-related changes in BM variables might be important,possibly affecting the interpretation of the results.</p>\n </section>\n \n <section>\n \n <h3> Objectives</h3>\n \n <p>Establish BM cytologic reference intervals (RIs) and association of age and sex on these findings, in healthy juvenile and young adult cats.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>BM aspirates of cats deemed healthy based on history and clinical, CBC, serum chemistry findings, and negative retrovirus serology were obtained and examined cytologically. The examination included a 1000-nucleated differential cell count and cell morphologic assessment. RIs were calculated using parametric, robust, and nonparametric methods. The cytologic findings were examined for associations with sex and age.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The study included 40 cats (females, 22 [55%]; males, 18 [45%]) with a median age of 1.5 years (range 0.5–5). Most calculated RIs were similar to those previously reported. BM plasma cell and monocyte counts were weakly and positively correlated with age (<i>r</i><sub>s</sub>, .312 and .373, respectively; <i>P</i> < .05). Metarubricytes were higher infemales (mean, 25.1%; SD, 6.0%) than males (mean, 21.2%; SD, 6.0%; <i>P</i> < .05).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The BM differential cell counts determined in this study can serve as RIs for cats aged 0.5–5 years.</p>\n </section>\n </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary clinical pathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/vcp.13349","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

The current bone marrow (BM) reference intervals (RI) are based on a limited number of cats. Age-related changes in BM variables might be important,possibly affecting the interpretation of the results.

Objectives

Establish BM cytologic reference intervals (RIs) and association of age and sex on these findings, in healthy juvenile and young adult cats.

Methods

BM aspirates of cats deemed healthy based on history and clinical, CBC, serum chemistry findings, and negative retrovirus serology were obtained and examined cytologically. The examination included a 1000-nucleated differential cell count and cell morphologic assessment. RIs were calculated using parametric, robust, and nonparametric methods. The cytologic findings were examined for associations with sex and age.

Results

The study included 40 cats (females, 22 [55%]; males, 18 [45%]) with a median age of 1.5 years (range 0.5–5). Most calculated RIs were similar to those previously reported. BM plasma cell and monocyte counts were weakly and positively correlated with age (r_s, .312 and .373, respectively; P < .05). Metarubricytes were higher infemales (mean, 25.1%; SD, 6.0%) than males (mean, 21.2%; SD, 6.0%; P < .05).

Conclusions

The BM differential cell counts determined in this study can serve as RIs for cats aged 0.5–5 years.

查看原文本刊更多论文

幼猫和青年猫骨髓细胞的参考区间骨髓细胞学

背景目前的骨髓（BM）参考区间（RI）是基于数量有限的猫。方法采集根据病史和临床、全血细胞计数、血清化学检查结果以及逆转录病毒血清学检查结果为阴性的健康猫的骨髓穿刺液并进行细胞学检查。检查包括 1000 个有核差异细胞计数和细胞形态学评估。采用参数法、稳健法和非参数法计算 RI。研究包括 40 只猫（雌性 22 只 [55%]；雄性 18 只 [45%]），中位年龄为 1.5 岁（0.5-5 岁不等）。大多数计算出的 RI 与之前报告的结果相似。血浆细胞和单核细胞计数与年龄呈弱正相关（rs，分别为.312和.373；P <.05）。雌性（平均值为 25.1%；标清值为 6.0%）的元润滑细胞高于雄性（平均值为 21.2%；标清值为 6.0%；P <.05）。结论本研究确定的基础细胞差异计数可作为 0.5-5 岁猫的 RI。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.