Capsaicin attenuates Porphyromonas gingivalis-suppressed osteogenesis of periodontal ligament stem cells via regulating mitochondrial function and activating PI3K/AKT/mTOR pathway

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research Pub Date : 2024-05-03 DOI:10.1111/jre.13252

Weijia Wang, Zhiyan Zhou, Tian Ding, Susu Feng, Hongrui Liu, Mengmeng Liu, Shaohua Ge

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引用次数: 0

Abstract

Background and Objective

Prevention of periodontal bone resorption triggered by Porphyromonas gingivalis (P. gingivalis) is crucial for dental stability. Capsaicin, known as the pungent ingredient of chili peppers, can activate key signaling molecules involved in osteogenic process. However, the effect of capsaicin on osteogenesis of periodontal ligament stem cells (PDLSCs) under inflammation remains elusive.

Methods

P. gingivalis culture suspension was added to mimic the inflammatory status after capsaicin pretreatment. The effects of capsaicin on the osteogenesis of PDLSCs, as well as mitochondrial morphology, Ca²⁺ level, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and osteogenesis-regulated protein expression levels were analyzed. Furthermore, a mouse experimental periodontitis model was established to evaluate the effect of capsaicin on alveolar bone resorption and the expression of osteogenesis-related proteins.

Results

Under P. gingivalis stimulation, capsaicin increased osteogenesis of PDLSCs. Not surprisingly, capsaicin rescued the damage to mitochondrial morphology, decreased the concentration of intracellular Ca²⁺ and ROS, enhanced MMP and activated phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. The in vivo results showed that capsaicin significantly attenuated alveolar bone loss and augmented the expression of bone associated proteins.

Conclusion

Capsaicin increases osteogenesis of PDLSCs under inflammation and reduces alveolar bone resorption in mouse experimental periodontitis.

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辣椒素通过调节线粒体功能和激活 PI3K/AKT/mTOR 通路，减轻牙龈卟啉单胞菌抑制的牙周韧带干细胞成骨作用

背景和目的预防牙龈卟啉单胞菌（P. gingivalis）引发的牙周骨吸收对牙齿的稳定性至关重要。辣椒素是辣椒中的刺激性成分，可激活参与成骨过程的关键信号分子。然而，辣椒素对炎症条件下牙周韧带干细胞（PDLSCs）成骨过程的影响仍不明确。分析了辣椒素对 PDLSCs 骨生成的影响，以及线粒体形态、Ca2+水平、活性氧（ROS）、线粒体膜电位（MMP）和骨生成调控蛋白的表达水平。此外，还建立了小鼠实验性牙周炎模型，以评估辣椒素对牙槽骨吸收和成骨相关蛋白表达的影响。令人惊讶的是，辣椒素能修复线粒体形态的损伤、降低细胞内 Ca2+ 和 ROS 的浓度、增强 MMP 和激活磷脂酰肌醇 3- 激酶（PI3K）/蛋白激酶 B（AKT）/哺乳动物雷帕霉素靶标（mTOR）通路。体内研究结果表明，辣椒素可明显减少牙槽骨流失，并增加骨相关蛋白的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.