A facile and scalable method to synthesize PEGylated PDMAEMA for gene delivery

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biopolymers Pub Date : 2024-05-02 DOI:10.1002/bip.23584

Jie Dou, Shupei Yu, Yuanwei Zhang

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Abstract

In recent years, cationic polymer vectors have been viewed as a promising method for delivering nucleic acids. With the advancement of synthetic polymer chemistry, we can control chemical structures and properties to enhance the efficacy of gene delivery. Herein, a facile, cost-effective, and scalable method was developed to synthesize PEGylated PDMAEMA polymers (PEO-PDMAEMA-PEO), where PEGylation could enable prolonged polyplexes circulation time in the blood stream. Two polymers of different molecular weights were synthesized, and polymer/eGFP polyplexes were prepared and characterized. The correlation between polymers' molecular weight and physicochemical properties (size and zeta potential) of polyplexes was investigated. Lipofectamine 2000, a commercial non-viral transfection reagent, was used as a standard control. PEO-PDMAEMA-PEO with higher molecular weight exhibited slightly better transfection efficiency than Lipofectamine 2000, and the cytotoxicity study proved that it could function as a safe gene vector. We believe that PEO-PDMAEMA-PEO could serve as a model to investigate more potential in the gene delivery area.

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用于基因递送的 PEG 化 PDMAEMA 的简便、可扩展合成方法

近年来，阳离子聚合物载体被视为一种很有前景的核酸递送方法。随着合成聚合物化学的发展，我们可以通过控制化学结构和性质来提高基因递送的效果。本文开发了一种简便、经济、可扩展的方法来合成 PEG 化 PDMAEMA 聚合物（PEO-PDMAEMA-PEO），其中 PEG 化可以延长多聚体在血流中的循环时间。我们合成了两种不同分子量的聚合物，并制备和表征了聚合物/eGFP 多聚体。研究了聚合物分子量与多聚体理化性质（大小和 zeta 电位）之间的相关性。以商用非病毒转染试剂 Lipofectamine 2000 作为标准对照。分子量更高的 PEO-PDMAEMA-PEO 的转染效率略高于 Lipofectamine 2000，细胞毒性研究证明它可以作为一种安全的基因载体。我们相信，PEO-PDMAEMA-PEO 可以作为一个模型，在基因递送领域发挥更大的潜力。

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来源期刊

Biopolymers 生物-生化与分子生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Founded in 1963, Biopolymers publishes strictly peer-reviewed papers examining naturally occurring and synthetic biological macromolecules. By including experimental and theoretical studies on the fundamental behaviour as well as applications of biopolymers, the journal serves the interdisciplinary biochemical, biophysical, biomaterials and biomedical research communities.