Magnetic Seeding of SPIO-BMSCs Into a Biphasic Scaffold Can Promote Tendon-Bone Healing After Rotator Cuff Repair

Chi Zhang, Jia-Le Jin, Cong-Hui Zhou, Cheng-Xing Ruan, Peng-Fei Lei, You-Zhi Cai
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Abstract

Background:The tendon-bone interface (TBI) in the rotator cuff has a poor intrinsic capacity for healing, which increases the risk of retear after rotator cuff repair (RCR). However, facilitating regeneration of the TBI still remains a great clinical challenge. Herein, the authors established a novel strategy based on magnetic seeding to enhance the TBI regeneration.Hypothesis:Magnetic seeding bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide (SPIO-BMSCs) into a biphasic scaffold can promote tendon-bone healing after RCR.Study Design:Controlled laboratory study.Methods:BMSCs were labeled with SPIOs. Prussian blue staining, CCK-8 tests, Western blot, and quantitative reverse transcription polymerase chain reaction (PCR) were used to determine the optimal effect concentration of SPIOs on cell bioactivities and abilities. Then SPIO-BMSCs were magnetically seeded into a biphasic scaffold under a magnetic field. The seeding efficacy was assessed by a scanning electron microscope, and the potential mechanism in chondrogenic differentiation after seeding SPIO-BMSCs into the scaffold was evaluated by Western blot and PCR. Furthermore, the effect of SPIO-BMSC/biphasic scaffold on tendon-bone healing after RCR using a rat model was examined using histological analysis, enzyme-linked immunosorbent assay, and biomechanical evaluation.Results:BMSCs labeled with 100 μg/mL SPIO had no effect on cell bioactivities and the ability of chondrogenic differentiation. SPIO-BMSCs were magnetically seeded into a biphasic scaffold, which offered a high seeding efficacy to enhance chondrogenic differentiation of SPIO-BMSCs via the CDR1as/miR-7/FGF2 pathway for TBI formation in vitro. Furthermore, in vivo application of the biphasic scaffold with magnetically seeded SPIO-BMSCs showed their regenerative potential, indicating that they could significantly accelerate and promote TBI healing with superior biomechanical properties after RCR in a rat rotator cuff tear model.Conclusion:Magnetically seeding SPIO-BMSCs into a biphasic scaffold enhanced seeding efficacy to promote cell distribution and condensation. This construct enhanced the chondrogenesis process via the CDR1as/miR-7/FGF2 pathway and further promoted tendon-bone healing after RCR in a rat rotator cuff tear model.Clinical Relevance:This study provides an alternative strategy for improving TBI healing after RCR.
将 SPIO-BMSCs 磁性植入双相支架可促进肩袖修复后的肌腱-骨愈合
背景:肩袖肌腱骨界面(TBI)的内在愈合能力较差,这增加了肩袖修复术(RCR)后再次撕裂的风险。然而,促进 TBI 的再生仍是一项巨大的临床挑战。假设:将标记有超顺磁性氧化铁的骨髓间充质干细胞(SPIO-BMSCs)磁性播种到双相支架中,可促进 RCR 后肌腱骨的愈合。采用普鲁士蓝染色、CCK-8检测、Western印迹和定量反转录聚合酶链反应(PCR)确定SPIOs对细胞生物活性和能力的最佳影响浓度。然后在磁场下将 SPIO-BMSCs 磁性播种到双相支架中。扫描电子显微镜评估了播种效果,Western blot 和 PCR 评估了将 SPIO-BMSCs 播种到支架后软骨分化的潜在机制。此外,还通过组织学分析、酶联免疫吸附试验和生物力学评估,研究了 SPIO-BMSC/ 双相支架对大鼠模型 RCR 后肌腱骨愈合的影响。将SPIO-BMSCs磁性播种到双相支架中,该支架具有很高的播种效率,可通过CDR1as/miR-7/FGF2途径提高SPIO-BMSCs的软骨分化能力,从而在体外形成TBI。此外,磁性播种 SPIO-BMSCs 的双相支架在体内的应用显示了其再生潜力,表明在大鼠肩袖撕裂模型中,磁性播种 SPIO-BMSCs 可显著加速和促进 TBI 愈合,并在 RCR 后具有优异的生物力学特性。临床意义:这项研究为改善大鼠肩袖撕裂模型 RCR 后的 TBI 愈合提供了另一种策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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