Microwave-assisted and methanol/acetic acid-free method for rapid staining of proteins in SDS-PAGE gels

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-05-01 DOI:10.1016/j.ab.2024.115553

Jinzhong Wang , Zhaoyang Wang , Wei Zhao , Ying Wang

引用次数: 0

Abstract

We describe a microwave-assisted, methanol and acetic acid-free, inexpensive method for rapid staining of SDS-PAGE proteins. Only citric acid, benzoic acid, and Coomassie brilliant blue G-250 (CBG) were used. Microwave irradiation reduced the detection duration, and proteins in a clear background were visualized within 30 min of destaining, after 2 min of fixing and 12 min of staining. By using this protocol, comparable band intensities were obtained to the conventional methanol/acetic acid method.

Abstract Image

查看原文本刊更多论文

微波辅助且不含甲醇/醋酸的 SDS-PAGE 凝胶蛋白质快速染色法

我们介绍了一种微波辅助、不含甲醇和醋酸、成本低廉的 SDS-PAGE 蛋白质快速染色方法。只使用了柠檬酸、苯甲酸和库马西亮蓝 G-250 (CBG)。微波照射缩短了检测时间，在去渍 30 分钟、固定 2 分钟和染色 12 分钟后，背景清晰的蛋白质即可显现。使用该方案可获得与传统甲醇/乙酸法相当的条带强度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.