On-demand expansion fluorescence and photoacoustic microscopy (ExFLPAM)

IF 7.1 1区医学 Q1 ENGINEERING, BIOMEDICAL

Photoacoustics Pub Date : 2024-04-29 DOI:10.1016/j.pacs.2024.100610

Xuan Mu , Chenshuo Ma , Xuan Mei , Junlong Liao , Rebecca Bojar , Sizhe Kuang , Qiangzhou Rong , Junjie Yao , Yu Shrike Zhang

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引用次数: 0

Abstract

Expansion microscopy (ExM) is a promising technology that enables nanoscale imaging on conventional optical microscopes by physically magnifying the specimens. Here, we report the development of a strategy that enables i) on-demand labeling of subcellular organelles in live cells for ExM through transfection of fluorescent proteins that are well-retained during the expansion procedure; and ii) non-fluorescent chromogenic color-development towards efficient bright-field and photoacoustic imaging in both planar and volumetric formats, which is applicable to both cultured cells and biological tissues. Compared to the conventional ExM methods, our strategy provides an expanded toolkit, which we term as expansion fluorescence and photoacoustic microscopy (ExFLPAM), by allowing on-demand fluorescent protein labeling of cultured cells, as well as non-fluorescent absorption contrast-imaging of biological samples.

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按需扩展荧光和光声显微镜（ExFLPAM）

膨胀显微镜（ExM）是一种前景广阔的技术，它通过物理放大标本，在传统光学显微镜上实现纳米级成像。在此，我们报告了一种策略的开发情况：i）通过转染荧光蛋白，在活细胞中按需标记亚细胞器，以用于 ExM；ii）非荧光色原显色，实现平面和体积格式的高效明视野和光声成像，该策略适用于培养细胞和生物组织。与传统的 ExM 方法相比，我们的策略提供了一个扩展工具包，我们称之为扩展荧光和光声显微镜（ExFLPAM），它允许按需对培养细胞进行荧光蛋白标记，以及对生物样本进行非荧光吸收对比成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photoacoustics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

11.40

自引率

16.50%

发文量

审稿时长

53 days

期刊介绍： The open access Photoacoustics journal (PACS) aims to publish original research and review contributions in the field of photoacoustics-optoacoustics-thermoacoustics. This field utilizes acoustical and ultrasonic phenomena excited by electromagnetic radiation for the detection, visualization, and characterization of various materials and biological tissues, including living organisms. Recent advancements in laser technologies, ultrasound detection approaches, inverse theory, and fast reconstruction algorithms have greatly supported the rapid progress in this field. The unique contrast provided by molecular absorption in photoacoustic-optoacoustic-thermoacoustic methods has allowed for addressing unmet biological and medical needs such as pre-clinical research, clinical imaging of vasculature, tissue and disease physiology, drug efficacy, surgery guidance, and therapy monitoring. Applications of this field encompass a wide range of medical imaging and sensing applications, including cancer, vascular diseases, brain neurophysiology, ophthalmology, and diabetes. Moreover, photoacoustics-optoacoustics-thermoacoustics is a multidisciplinary field, with contributions from chemistry and nanotechnology, where novel materials such as biodegradable nanoparticles, organic dyes, targeted agents, theranostic probes, and genetically expressed markers are being actively developed. These advanced materials have significantly improved the signal-to-noise ratio and tissue contrast in photoacoustic methods.