Salivary microbiome and MRP‐8/14 levels in children with gingivitis, healthy children, and their mothers

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Alice Blufstein, Natasa Pejcic, Kathrin Spettel, Bela Hausmann, David Seki, Tugba Ertekin, Julia Hinrichs‐Priller, Sarra Altner, Marion Nehr, Katrin Bekes, Athanasios Makristathis, Oleh Andrukhov
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引用次数: 0

Abstract

BackgroundGingivitis is the most common form of periodontal disease among children and adolescents and is associated with disrupted host–microbiome homeostasis. Family is an important factor influencing the prevalence of gingivitis. In the present study, we investigated the salivary microbiome, oral hygiene habits, and the salivary level of myeloid‐related protein (MRP)‐8/14 in children aged 7–12 years with gingivitis, periodontally healthy children, and their mothers.MethodsThis study included 24 children with gingivitis (including four sibling pairs) and 22 periodontally healthy children (including two sibling pairs) and their mothers. The whole saliva was collected, DNA was extracted, the variable V3–V4 region of the eubacterial 16S ribosomal RNA gene was amplified, and sample library preparation was performed according to the Illumina protocol. The salivary levels of MRP‐8/14 were analyzed by ELISA.ResultsAlpha diversity of the salivary microbiome was considerably higher in gingivitis children and mothers of gingivitis children compared to healthy children and their mothers, respectively. Significant differences in beta diversity between healthy and gingivitis children, healthy children and their mothers, and gingivitis children and their mothers were detected. Overall, the number of common core amplicon sequence variants between children and their own mothers was significantly higher than between children and other mothers. The salivary MRP‐8/14 levels in children with gingivitis were significantly higher compared to healthy children; a similar tendency was also mentioned for mothers.ConclusionOur study underlines the importance of family as an essential factor influencing oral health.
牙龈炎儿童、健康儿童及其母亲的唾液微生物群和 MRP-8/14 水平
背景牙龈炎是儿童和青少年中最常见的牙周疾病,与宿主-微生物组平衡紊乱有关。家庭是影响牙龈炎发病率的一个重要因素。本研究调查了 7-12 岁牙龈炎儿童、牙周健康儿童及其母亲的唾液微生物组、口腔卫生习惯和唾液中髓样相关蛋白(MRP)-8/14 的水平。采集全唾液,提取DNA,扩增真细菌16S核糖体RNA基因的可变V3-V4区,并按照Illumina协议进行样本库制备。结果与健康儿童及其母亲相比,牙龈炎儿童及其母亲的唾液微生物组的α多样性要高得多。健康儿童和牙龈炎儿童、健康儿童及其母亲以及牙龈炎儿童及其母亲之间的β多样性存在显著差异。总体而言,儿童与其母亲之间的共同核心扩增片段序列变异数明显高于儿童与其他母亲之间的共同核心扩增片段序列变异数。与健康儿童相比,牙龈炎儿童唾液中的 MRP-8/14 水平明显更高;母亲也有类似的趋势。
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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