Yuxin Zhao , Zhanyi Yao , Vedika Desai , Dan Chen , Zengyi Shao
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引用次数: 0
Abstract
Fat-soluble antioxidants play a vital role in protecting the body against oxidative stress and damage. The rapid advancements in metabolic engineering and synthetic biology have offered a promising avenue for economically producing fat-soluble antioxidants by engineering microbial chassis. This review provides an overview of the recent progress in engineering yeast microbial factories to produce three main groups of lipophilic antioxidants: carotenoids, vitamin E, and stilbenoids. In addition to discussing the classic strategies employed to improve precursor availability and alleviate carbon flux competition, this review delves deeper into the innovative approaches focusing on enzyme engineering, product sequestration, subcellular compartmentalization, multistage fermentation, and morphology engineering. We conclude the review by highlighting the prospects of microbial engineering for lipophilic antioxidant production.
脂溶性抗氧化剂在保护人体免受氧化压力和损害方面发挥着重要作用。代谢工程和合成生物学的飞速发展为通过微生物底盘工程经济地生产脂溶性抗氧化剂提供了广阔的前景。本综述概述了在酵母微生物工厂工程化生产三大类亲脂性抗氧化剂（类胡萝卜素、维生素 E 和类芪）方面的最新进展。除了讨论为提高前体可用性和缓解碳通量竞争而采用的经典策略外，本综述还深入探讨了创新方法，重点是酶工程、产物螯合、亚细胞区隔、多级发酵和形态工程。最后，我们强调了亲脂性抗氧化剂生产的微生物工程前景。
期刊介绍：
Current Opinion in Biotechnology (COBIOT) is renowned for publishing authoritative, comprehensive, and systematic reviews. By offering clear and readable syntheses of current advances in biotechnology, COBIOT assists specialists in staying updated on the latest developments in the field. Expert authors annotate the most noteworthy papers from the vast array of information available today, providing readers with valuable insights and saving them time.
As part of the Current Opinion and Research (CO+RE) suite of journals, COBIOT is accompanied by the open-access primary research journal, Current Research in Biotechnology (CRBIOT). Leveraging the editorial excellence, high impact, and global reach of the Current Opinion legacy, CO+RE journals ensure they are widely read resources integral to scientists' workflows.
COBIOT is organized into themed sections, each reviewed once a year. These themes cover various areas of biotechnology, including analytical biotechnology, plant biotechnology, food biotechnology, energy biotechnology, environmental biotechnology, systems biology, nanobiotechnology, tissue, cell, and pathway engineering, chemical biotechnology, and pharmaceutical biotechnology.
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