Structure and composition of microbial communities in the water column from Southern Gulf of Mexico and detection of putative hydrocarbon-degrading microorganisms

IF 3.6 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2024-05-01 DOI:10.1111/1758-2229.13264

Sonia S. Valencia-Agami, Daniel Cerqueda-García, Abril M. Gamboa-Muñoz, M. Leopoldina Aguirre-Macedo, José Q. García-Maldonado

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Abstract

This study assessed the bacterioplankton community and its relationship with environmental variables, including total petroleum hydrocarbon (TPH) concentration, in the Yucatan shelf area of the Southern Gulf of Mexico. Beta diversity analyses based on 16S rRNA sequences indicated variations in the bacterioplankton community structure among sampling sites. PERMANOVA indicated that these variations could be mainly related to changes in depth (5 to 180 m), dissolved oxygen concentration (2.06 to 5.93 mg L⁻¹), and chlorophyll-a concentration (0.184 to 7.65 mg m³). Moreover, SIMPER and one-way ANOVA analyses showed that the shifts in the relative abundances of Synechococcus and Prochlorococcus were related to changes in microbial community composition and chlorophyll-a values. Despite the low TPH content measured in the studied sites (0.01 to 0.86 μL L⁻¹), putative hydrocarbon-degrading bacteria such as Alteromonas, Acinetobacter, Balneola, Erythrobacter, Oleibacter, Roseibacillus, and the MWH-UniP1 aquatic group were detected. The relatively high copy number of the alkB gene detected in the water column by qPCR and the enrichment of hydrocarbon-degrading bacteria obtained during lab crude oil tests exhibited the potential of bacterioplankton communities from the Yucatan shelf to respond to potential hydrocarbon impacts in this important area of the Gulf Mexico.

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墨西哥湾南部水体微生物群落的结构和组成以及推定碳氢化合物降解微生物的检测

本研究评估了墨西哥湾南部尤卡坦大陆架地区的浮游细菌群落及其与环境变量（包括总石油碳氢化合物（TPH）浓度）之间的关系。基于 16S rRNA 序列的 Beta 多样性分析表明，不同采样地点的浮游细菌群落结构存在差异。PERMANOVA 分析表明，这些变化主要与深度（5 至 180 米）、溶解氧浓度（2.06 至 5.93 毫克/升）和叶绿素-a 浓度（0.184 至 7.65 毫克/立方米）的变化有关。此外，SIMPER 和单因子方差分析表明，Synechococcus 和 Prochlorococcus 相对丰度的变化与微生物群落组成和叶绿素-a 值的变化有关。尽管研究地点测得的 TPH 含量较低（0.01 至 0.86 μL L-1），但仍检测到了推定的碳氢化合物降解细菌，如 Alteromonas、Acinetobacter、Balneola、Erythrobacter、Oleibacter、Roseibacillus 和 MWH-UniP1 水生组。通过 qPCR 在水体中检测到的相对较高的 alkB 基因拷贝数，以及在实验室原油测试中获得的碳氢化合物降解细菌的富集，表明尤卡坦大陆架的浮游细菌群落有可能对墨西哥湾这一重要地区潜在的碳氢化合物影响做出反应。

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来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.