Xia Xu, Sana Asghar, Changjiang Hu, Feng-Ling Si, Fei Tao, Bin Chen
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引用次数: 0
Abstract
Pyrethroid resistance has become one of the largest obstacles to mosquito control, and Anopheles sinensis is the main malaria vector in China and southeast countries. miRNAs play important roles in many biological processes in insects; however, the diversity and regulation of miRNAs associated with pyrethroid resistance are still little understood at the whole-genome level. This study performed the sequencing and analysis of miRNAs relative to pyrethroid resistance in An. sinensis for the first time, and identified 328 miRNAs, of which 247 are new. A total of 39 miRNAs are identified to be significantly downregulated commonly in all three pyrethroid-resistant populations investigated in comparison to the susceptible strain. There are 7475 genes to be predicted to be targeted by the 39 significantly down-regulated miRNAs, and they were enriched to seven pathways. The regulation of Transferrin by asi-miR-87 was verified using dual-luciferase, and the asi-miR-87 might be involved in P450 pyrethroid detoxification with the affection of iron ion transportation and synthesis through Transferrin. In addition, nine miRNAs were identified to be positively relative to eight genes. This is the first systematic study on the diversity and regulation of miRNAs associated with insecticide resistance at the whole-genome level, and provides an information frame for the understanding of miRNAs and their function in insecticide resistance.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.