{"title":"Enhancing microspore embryogenesis in diploid potato (Solanum tuberosum L. ssp. tuberosum) germplasm","authors":"Alison M.R. Ferrie, Jennifer M. Brost","doi":"10.1007/s11240-024-02768-8","DOIUrl":null,"url":null,"abstract":"<p>Doubled haploidy (DH) technology has been utilized in cultivated tetraploid potato (<i>Solanum tuberosum</i> L. ssp. <i>tuberosum</i>) to accelerate crop improvement; however very little work has been done with the diploid species. Experiments were undertaken to improve microspore embryogenic response in the diploid germplasm. Several factors influencing embryogenic responses were evaluated. An increase in calcium nitrate, a reduction in the plant growth regulators 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA), as well as an incubation temperature of 28 °C resulted in an increase in callus production and, in some cases, embryo-like structures. Validation of the modified protocol was conducted with both diploid and tetraploid potato germplasm with responses from both diploid and tetraploid. Monoploid and di-haploid plants were also regenerated from these microspore-derived calli.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"23 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell, Tissue and Organ Culture","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02768-8","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Doubled haploidy (DH) technology has been utilized in cultivated tetraploid potato (Solanum tuberosum L. ssp. tuberosum) to accelerate crop improvement; however very little work has been done with the diploid species. Experiments were undertaken to improve microspore embryogenic response in the diploid germplasm. Several factors influencing embryogenic responses were evaluated. An increase in calcium nitrate, a reduction in the plant growth regulators 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA), as well as an incubation temperature of 28 °C resulted in an increase in callus production and, in some cases, embryo-like structures. Validation of the modified protocol was conducted with both diploid and tetraploid potato germplasm with responses from both diploid and tetraploid. Monoploid and di-haploid plants were also regenerated from these microspore-derived calli.
提高二倍体马铃薯（Solanum tuberosum L. ssp. tuberosum）种质的小孢子胚胎发生率
双倍单倍体（DH）技术已被用于栽培四倍体马铃薯（Solanum tuberosum L. ssp. tuberosum），以加速作物改良；但在二倍体物种方面所做的工作却很少。为了改善二倍体种质的小孢子胚胎发生反应，我们进行了实验。对影响胚胎发生反应的几个因素进行了评估。硝酸钙的增加、植物生长调节剂 6-苄基腺嘌呤（BA）和α-萘乙酸（NAA）的减少以及 28 °C 的孵育温度都导致胼胝体产量的增加，在某些情况下还出现了胚状结构。用二倍体和四倍体马铃薯种质对修改后的方案进行了验证，二倍体和四倍体都做出了反应。这些小孢子衍生的胼胝体还能再生出单倍体和二倍体植株。
期刊介绍：
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
