{"title":"Lipid Transfer Protein Genes Are Differently Expressed in Rat Soleus Muscle at Early and Later Periods of Muscle Disuse","authors":"I. G. Bryndina, V. V. Skurygin, V. V. Protopopov","doi":"10.1134/s002209302402011x","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>This work reports analysis of lipid transfer protein (LTPs)\nmRNA levels in rat soleus muscle after 12-h and 14-day hindlimb\nsuspension (HS). The LTP genes of interest were as follows: ceramide\ntransporter 1 (<i>CERT1</i>), ceramide-1-phosphate\ntransporter protein (<i>CPTP</i>),\nsphingosine-1-phosphate transporter (Protein spinster homolog 2—<i>SPNS2</i>), four-phosphate adaptor protein\n2 (<i>FAPP2</i>) transferring glucosylceramide,\nand oxysterol-binding proteins <i>OSBP</i> and <i>ORP4</i> (family I of OSBP-related proteins).\nLTP mRNA expression was analyzed using RT-PCR. Early changes in\nthe expression of LTP genes after 12-h HS were characterized by\nthe dramatic decrease in mRNA content of <i>CERT1</i>, <i>CPTP</i>, <i>SPNS2</i> and <i>ORP4</i> genes. In contrast, the expression\nof the <i>FAPP2</i> gene was increased,\nwhereas <i>OSBP</i> mRNA content did\nnot change as compared to the control level. After the 14-day HS\nonly <i>CERT1</i> and <i>CPTP</i> mRNA levels remained decreased\nwhile expression of other LTP genes did not significantly differ from\nthe control values. Clomipramine treatment did not affect sphingolipid\ntransporters and <i>OSBPs</i> at the\nearly period of HS, but notably enhanced the expression of <i>CERT1</i>, <i>CPTP</i>, <i>FAPP2</i>, and both <i>OSBPs</i> genes\nin soleus muscle of rats subjected to 14-day unloading. Our data\nindicate that short-term and long-term muscle unloading differently\naffects the expression of genes encoding LTPs for sphingolipid and\ncholesterol intracellular transport. Only ceramide and ceramide-1-phosphate transporters\nwere decreased at both periods of disuse. Taking into account our\nprevious data about acid sphingomyelinase (aSMase) and ceramide\nup-regulation in the unloaded soleus muscle, as well as a normalizing\neffect of clomipramine on both high ceramide level and low expression\nof its transporters at 14-day HS, we can assume that there must\nbe a feedback mechanism in regulation of <i>CERT1</i> and <i>CPTP</i> genes expression in case of\nceramide accumulation caused by disuse.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"27 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s002209302402011x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This work reports analysis of lipid transfer protein (LTPs)
mRNA levels in rat soleus muscle after 12-h and 14-day hindlimb
suspension (HS). The LTP genes of interest were as follows: ceramide
transporter 1 (CERT1), ceramide-1-phosphate
transporter protein (CPTP),
sphingosine-1-phosphate transporter (Protein spinster homolog 2—SPNS2), four-phosphate adaptor protein
2 (FAPP2) transferring glucosylceramide,
and oxysterol-binding proteins OSBP and ORP4 (family I of OSBP-related proteins).
LTP mRNA expression was analyzed using RT-PCR. Early changes in
the expression of LTP genes after 12-h HS were characterized by
the dramatic decrease in mRNA content of CERT1, CPTP, SPNS2 and ORP4 genes. In contrast, the expression
of the FAPP2 gene was increased,
whereas OSBP mRNA content did
not change as compared to the control level. After the 14-day HS
only CERT1 and CPTP mRNA levels remained decreased
while expression of other LTP genes did not significantly differ from
the control values. Clomipramine treatment did not affect sphingolipid
transporters and OSBPs at the
early period of HS, but notably enhanced the expression of CERT1, CPTP, FAPP2, and both OSBPs genes
in soleus muscle of rats subjected to 14-day unloading. Our data
indicate that short-term and long-term muscle unloading differently
affects the expression of genes encoding LTPs for sphingolipid and
cholesterol intracellular transport. Only ceramide and ceramide-1-phosphate transporters
were decreased at both periods of disuse. Taking into account our
previous data about acid sphingomyelinase (aSMase) and ceramide
up-regulation in the unloaded soleus muscle, as well as a normalizing
effect of clomipramine on both high ceramide level and low expression
of its transporters at 14-day HS, we can assume that there must
be a feedback mechanism in regulation of CERT1 and CPTP genes expression in case of
ceramide accumulation caused by disuse.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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