Facile engineering of aptamer-coupled silk fibroin encapsulated myogenic gold nanocomposites: investigation of antiproliferative activity and apoptosis induction

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2024-04-27 DOI:10.1007/s10529-024-03491-2

Poorni Kaliyappan Elayappan, Kavitha Kandasamy, Vadivukkarasi Sasikumar, Muruganantham Bharathi, Abdurahman Hajinur Hirad, Abdullah A. Alarfaj, Palanisamy Arulselvan, Ravindran Jaganathan, Rajeswari Ravindran, Jagadeesh Suriyaprakash, Indumathi Thangavelu

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Abstract

Nanocomposites selectively induce cancer cell death, holding potential for precise liver cancer treatment breakthroughs. This study assessed the cytotoxicity of gold nanocomposites (Au NCs) enclosed within silk fibroin (SF), aptamer (Ap), and the myogenic Talaromyces purpureogenus (TP) against a human liver cancer cell (HepG2). The ultimate product, Ap-SF-TP@Au NCs, results from a three-step process. This process involves the myogenic synthesis of TP@Au NCs derived from TP mycelial extract, encapsulation of SF on TP@Au NCs (SF-TP@Au NCs), and the conjugation of Ap within SF-TP@Au NCs. The synthesized NCs are analyzed by various characteristic techniques. Ap-SF-TP@Au NCs induced potential cell death in HepG2 cells but exhibited no cytotoxicity in non-cancerous cells (NIH3T3). The morphological changes in cells were examined through various biochemical staining methods. Thus, Ap-SF-TP@Au NCs emerge as a promising nanocomposite for treating diverse cancer cells.

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简便工程化的aptamer-耦合丝纤维蛋白包裹肌源性金纳米复合材料：抗增殖活性和细胞凋亡诱导研究

纳米复合材料可选择性地诱导癌细胞死亡，有望为肝癌的精确治疗带来突破。本研究评估了包裹在蚕丝纤维蛋白（SF）、aptamer（Ap）和致霉菌Talaromyces purpureogenus（TP）中的金纳米复合材料（Au NCs）对人类肝癌细胞（HepG2）的细胞毒性。最终产品 Ap-SF-TP@Au NCs 由三个步骤组成。该过程包括从 TP 菌丝提取物中提取 TP@Au NCs 的肌源性合成、在 TP@Au NCs 上封装 SF（SF-TP@Au NCs）以及在 SF-TP@Au NCs 内共轭 Ap。通过各种特征技术对合成的 NCs 进行了分析。Ap-SF-TP@Au NCs 可诱导 HepG2 细胞死亡，但对非癌细胞（NIH3T3）无细胞毒性。通过各种生化染色方法检测了细胞的形态变化。因此，Ap-SF-TP@Au NCs 是一种治疗各种癌细胞的前景广阔的纳米复合材料。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.