Telomere length determines the mitochondrial copy number in blastocyst-stage embryos

IF 3.9 3区生物学 Q2 CELL BIOLOGY

Mitochondrion Pub Date : 2024-04-24 DOI:10.1016/j.mito.2024.101887

Yuki Inoue, Sogo Aoki, Jun Ito, Shunsuke Hara, Komei Shirasuna, Hisataka Iwata

引用次数: 0

Abstract

Telomere length (TL) and mitochondrial DNA copy number (mt-cn) are associated with embryonic development. Here, we investigated the correlation between TL and mt-cn in bovine embryos to determine whether TL regulates mt-cn.

TL and mt-cn were closely correlated in embryos derived from six bulls. Treatment of embryos with a telomerase inhibitor (TMPyP) and siTERT shortened the TL and reduced mt-cn in blastocysts. RNA-sequencing of blastocysts developed with TMPyP revealed differentially expressed genes associated with transforming growth factor-β1 signaling and inflammation. In conclusion, TL regulates mt-cn in embryos.

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端粒长度决定囊胚期胚胎的线粒体拷贝数。

端粒长度（TL）和线粒体 DNA 拷贝数（mt-cn）与胚胎发育有关。在这里，我们研究了牛胚胎中端粒长度和线粒体 DNA 拷贝数的相关性，以确定端粒长度是否调节线粒体 DNA 拷贝数。用端粒酶抑制剂（TMPyP）和 siTERT 处理胚胎可缩短囊胚的 TL 并降低 mt-cn。对使用 TMPyP 培育的囊胚进行 RNA 测序发现，与转化生长因子-β1 信号传导和炎症相关的基因有不同表达。总之，TL 可调节胚胎中的 mt-cn。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mitochondrion 生物-细胞生物学

CiteScore

9.40

自引率

4.50%

发文量

审稿时长

13.6 weeks

期刊介绍： Mitochondrion is a definitive, high profile, peer-reviewed international research journal. The scope of Mitochondrion is broad, reporting on basic science of mitochondria from all organisms and from basic research to pathology and clinical aspects of mitochondrial diseases. The journal welcomes original contributions from investigators working in diverse sub-disciplines such as evolution, biophysics, biochemistry, molecular and cell biology, genetics, pharmacology, toxicology, forensic science, programmed cell death, aging, cancer and clinical features of mitochondrial diseases.