Inhibition and Disaggregation Effect of Flavonoid-derived Carbonized Polymer Dots on Protein Amyloid Aggregation

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-04-25 DOI:10.1016/j.colsurfb.2024.113928

Dexin Li, Sujuan Wang, Jiawei Dong, Jie Li, Xinnan Wang, Feng Liu, Xinwu Ba

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Abstract

In this research, four water-insoluble flavonoid compounds were utilized and reacted with arginine to prepare four carbonized polymer dots with good water-solubility in a hydrothermal reactor. Structural characterization demonstrated that the prepared carbonized polymer dots were classic core-shell structure. Effect of the prepared carbonized polymer dots on protein amyloid aggregation was further investigated using hen egg white lysozyme and human lysozyme as model protein in aqueous solution. All of the prepared carbonized polymer dots could retard the amyloid aggregation of hen egg white lysozyme and human lysozyme in a dose-depended manner. All measurements displayed that the inhibition ratio of luteolin-derived carbonized polymer dots (CPDs-1) was higher than that of the other three carbonized polymer dots under the same dosage. This result may be interpreted by the highest content of phenolic hydroxyl groups on the periphery. The inhibition ratio of CPDs-1 on hen egg white lysozyme and human lysozyme reached 88% and 83% at the concentration of 0.5 mg/mL, respectively. CPDs-1 also could disaggregate the formed mature amyloid fibrils into short aggregates.

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黄酮类碳化聚合物点对蛋白质淀粉样蛋白聚集的抑制和分解作用

本研究利用四种不溶于水的黄酮类化合物与精氨酸反应，在水热反应器中制备了四种具有良好水溶性的碳化聚合物点。结构表征表明，所制备的碳化聚合物点是典型的核壳结构。以水溶液中的母鸡蛋白溶菌酶和人溶菌酶为模型蛋白质，进一步研究了制备的碳化聚合物点对蛋白质淀粉样聚集的影响。所有制备的碳化聚合物点都能以剂量依赖的方式延缓母鸡蛋清溶菌酶和人溶菌酶的淀粉样聚集。所有测量结果均显示，在相同剂量下，木犀草素衍生的碳化聚合物点（CPDs-1）的抑制率高于其他三种碳化聚合物点。这一结果可能与外围酚羟基含量最高有关。浓度为 0.5 mg/mL 时，CPDs-1 对母鸡卵白溶菌酶和人溶菌酶的抑制率分别达到 88% 和 83%。CPDs-1 还能将已形成的成熟淀粉样纤维分解成短聚集体。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.