Constructing osteo-inductive bio-ink for 3D printing through hybridization of gelatin with maleic acid modified bacterial cellulose by regulating addition volumes of maleic acid solution

IF 20.2 Q1 MATERIALS SCIENCE, PAPER & WOOD
Xucai Wang , Dengxian Wu , Wei Liao , Yaxuan Liu , Wenhui Pei , Jixian Wang , Jiayu Gu , Peng Wang , Kai Lan , Caoxing Huang
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引用次数: 0

Abstract

Bacterial cellulose (BC) is an exopolysaccharide with unique properties that has been applied in various fields. However, the dense and intertwined nature of BC fibers limits its use in certain applications, including 3D printing scaffolds for bone regeneration. In this work, a controllable BC-based bio-ink for 3D printing was successfully prepared by modifying the neat BC through maleic acid (MA) treatment, aiming to promote bone tissue regeneration. To achieve homogeneous BC dispersions while preserving its crystalline and chemical properties, BC was modified by MA solution (60 %, w/V) with solid-liquid ratio from 1꞉5 to 1꞉50 (w/V) to obtain MA-BC dispersions. The analysis results from microstructure, chemical group, crystallinity, and wettability indicated that the BC/MA solution with ratio of 1꞉30 demonstrated the best pre-treatment performance to obtain MA-BC. Subsequently, by combining MA-BC with gelatin, we successfully formulated MA-BC-GEL gels with favorable rheological properties and compression modulus, which can be used as promising bio-inks for 3D bioprinting applications. In vitro tests demonstrated 1꞉30 MA-BC possessed excellent biocompatibility, a significant ability to express the alkaline phosphatase gene and osteogenic-related genes, and facilitated the formation of mineralized nodules. The utilization of this novel bio-ink in scaffold preparation for bone regeneration highlights the promising application of modified BC in bone tissue engineering field.

通过调节马来酸溶液的添加量,使明胶与马来酸改性细菌纤维素杂交,构建用于 3D 打印的骨诱导生物墨水
细菌纤维素(BC)是一种具有独特性质的外多糖,已被应用于多个领域。然而,BC 纤维的致密性和交织性限制了其在某些应用中的使用,包括用于骨再生的三维打印支架。在这项工作中,通过马来酸(MA)处理对纯净 BC 进行改性,成功制备了一种用于 3D 打印的可控 BC 生物墨水,旨在促进骨组织再生。为了在保留 BC 晶体和化学特性的同时使其分散均匀,采用固液比为 1꞉5 至 1꞉50 (w/V) 的马来酸溶液(60%,w/V)对 BC 进行改性,得到 MA-BC 分散体。从微观结构、化学基团、结晶度和润湿性等方面的分析结果表明,固液比为 1꞉30 的 BC/MA 溶液在获得 MA-BC 的预处理方面表现最佳。随后,通过将 MA-BC 与明胶结合,我们成功地配制出了具有良好流变特性和压缩模量的 MA-BC-GEL 凝胶,可作为有前景的生物墨水用于三维生物打印应用。体外测试表明,1꞉30 MA-BC 具有良好的生物相容性,能显著表达碱性磷酸酶基因和成骨相关基因,并能促进矿化结节的形成。利用这种新型生物墨水制备骨再生支架,凸显了改性 BC 在骨组织工程领域的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Bioresources and Bioproducts
Journal of Bioresources and Bioproducts Agricultural and Biological Sciences-Forestry
CiteScore
39.30
自引率
0.00%
发文量
38
审稿时长
12 weeks
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