Resistance phenotype and virulence potential of Leclercia adecarboxylata strains isolated from different sources.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-01 DOI:10.1099/mic.0.001457

Viviana Yescas-Zazueta, R. Rocha-Gracia, Cesar R González-Bonilla, J. F. Ayala-Zavala, Yessica Enciso-Martínez, Eder A. Carreón-León, Brigitte A González Corona, Dora Valencia, Manuel G. Ballesteros-Monrreal, Edwin Barrios-Villa

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Abstract

Introduction. Leclercia adecarboxylata is a member of Enterobacterales, often considered an opportunistic pathogen. Recent reports have highlighted L. adecarboxylata as an emerging pathogen harbouring virulence and resistance determinants.Gap statement. Little information exists on virulence and resistance determinants in L. adecarboxylata strains isolated from environmental, food, and clinical samples.Aim. To determine the presence of resistance and virulence determinants and plasmid features in L. adecarboxylata strains isolated from environmental, food, and clinical samples, as well as their phylogenetic relationship.Results. All strains tested showed resistance to β-lactams and quinolones but were sensitive to aminoglycosides and nitrofurans. However, even though fosfomycin resistance is considered a characteristic trait of L. adecarboxylata, the resistance phenotype was only observed in 50 % of the strains; bla TEM was the most prevalent BLEE gene (70 %), while the quinolone qnrB gene was observed in 60 % of the strains. Virulence genes were differentially observed in the strains, with adhesion-related genes being the most abundant, followed by toxin genes. Finally, all strains carried one to seven plasmid bands ranging from 7 to 125 kbps and harboured several plasmid addiction systems, such as ParDE, VagCD, and CcdAB in 80 % of the strains.Conclusions. L. adecarboxylata is an important emerging pathogen that may harbour resistance and virulence genes. Additionally, it has mobilizable genetic elements that may contribute to the dissemination of genetic determinants to other bacterial genera.

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从不同来源分离的 adecarboxylata Leclercia 菌株的抗性表型和毒力潜力。

简介adecarboxylata 是肠杆菌科的一个成员，通常被认为是一种机会性病原体。最近的报告强调，十八烷菌是一种新出现的病原体，具有毒力和抗药性决定因素。关于从环境、食品和临床样本中分离出的十八碳肠杆菌菌株的毒力和耐药性决定因素的信息很少。目的：确定从环境、食品和临床样本中分离出的 adecarboxylata 杆菌菌株中是否存在抗药性和毒力决定因素、质粒特征及其系统发育关系。所有检测菌株均对β-内酰胺类和喹诺酮类产生耐药性，但对氨基糖苷类和硝基呋喃类药物敏感。然而，尽管磷霉素抗性被认为是L. adecarboxylata的特征性性状，但只有50%的菌株观察到该抗性表型；bla TEM是最普遍的BLEE基因（70%），而喹诺酮类药物qnrB基因在60%的菌株中观察到。在菌株中观察到的毒力基因各不相同，粘附相关基因最多，其次是毒素基因。最后，所有菌株都携带 1 到 7 个质粒带，带宽从 7 kbps 到 125 kbps 不等，并带有多个质粒上瘾系统，如 ParDE、VagCD 和 CcdAB（80% 的菌株）。L.adecarboxylata是一种重要的新兴病原体，可能携带抗性基因和毒力基因。此外，它还具有可移动的遗传因子，可能有助于将遗传决定因子传播到其他细菌属。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.