The Effect of Chitosan Conjugates with Hydroxycinnamic Acids and Bacillus subtilis Bacteria on the Activity of Protective Proteins and Resistance of Potato Plants to Phytophthora infestans

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI:10.1134/S0003683824020194

L. G. Yarullina, G. F. Burkhanova, V. O. Tsvetkov, E. A. Cherepanova, A. V. Sorokan, E. A. Zaikina, I. S. Mardanshin, I. Y. Fatkullin, I. V. Maksimov, J. N. Kalatskaja, N. A. Yalouskaya, E. I. Rybinskaya

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Abstract

The effect of chitosan conjugates with caffeic (ChCA) and ferulic (ChFA) acids in combination with Bacillus subtilis bacteria on the transcriptional activity of PR protein genes and proteome changes in potato plants during infection with Phytophthora infestans (Mont.) de Bary was studied. Plants grown from mini tubers of the Udacha variety were sprayed with solutions of ChCA and ChFA, a suspension of B. subtilis bacteria strains 26D and 11 VM, and conjugates of ChCA of ChFA together with bacteria. At 3 days after treatment, some plants were infected with P. infestans. A decrease in the degree of development of the late blight pathogen on potato leaves in all treatment options was revealed. The maximum protective effect was manifested when plants were treated with the B. subtilis strain 26D bacteria in combination with conjugates of chitosan and oxycinnamic acids. The mechanisms of increasing the resistance of potato plants to P. infestans were associated with the activation of transcriptional activity of genes encoding the main protective protein (PR-1), chitinase (PR-3), thaumatin-like protein (PR-5), protease inhibitor (PR-6), peroxidase (PR-9), and ribonuclease (PR-10). The revealed activation of the expression of marker genes of systemic acquired resistance and induced systemic resistance under the influence of joint treatment of plants with B. subtilis and chitin conjugates with hydroxycinnamic acids indicates the synergistic development of protective reactions in potato plants in this variant. Using the method of two-dimensional electrophoresis of S. tuberosum leaf proteins followed by MALDI-TOF analysis 12 proteins were identified; their presence in the leaves differed depending on the variant of the experiment. In all treatment variants, serine–threonine protein phosphatase activity was suppressed, reflecting the development of the hypersensitivity reaction. Different variants of the experiment formed weakly expressed clusters, which indicates multiple mechanisms of regulation of the synthesis of protective proteins involved in the reaction to treatment with bacteria, chitosan conjugates, and infection with P. infestans.

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壳聚糖与羟基肉桂酸和枯草芽孢杆菌共轭物对马铃薯植株保护蛋白活性和抗病性的影响

在马铃薯植株感染 Phytophthora infestans (Mont.) de Bary 病毒期间，研究了壳聚糖与咖啡酸（ChCA）和阿魏酸（ChFA）共轭物以及枯草芽孢杆菌对 PR 蛋白基因转录活性和蛋白质组变化的影响。用 ChCA 和 ChFA 溶液、枯草芽孢杆菌菌株 26D 和 11 VM 的悬浮液以及 ChCA 和 ChFA 的共轭物与细菌一起喷洒从 Udacha 品种的微型块茎中生长出来的植物。处理 3 天后，一些植物感染了 P. infestans。结果表明，在所有处理方案中，马铃薯叶片上晚疫病病原体的发展程度都有所下降。用枯草杆菌菌株 26D 与壳聚糖和氧肉桂酸共轭物结合处理植物时，保护效果最大。提高马铃薯植株对 P. infestans 的抗性的机制与激活编码主要保护蛋白（PR-1）、几丁质酶（PR-3）、类潮霉素蛋白（PR-5）、蛋白酶抑制剂（PR-6）、过氧化物酶（PR-9）和核糖核酸酶（PR-10）的基因的转录活性有关。在枯草芽孢杆菌和羟基肉桂酸甲壳素共轭物共同处理植物的影响下，系统获得性抗性和诱导系统性抗性标记基因的表达被激活，这表明在该变体中马铃薯植物的保护性反应是协同发展的。通过对 S. tuberosum 叶片蛋白质进行二维电泳，然后进行 MALDI-TOF 分析，确定了 12 种蛋白质；它们在叶片中的存在因实验变体而异。在所有处理变体中，丝氨酸-苏氨酸蛋白磷酸酶活性都受到抑制，这反映了超敏反应的发展。实验的不同变体形成了弱表达群，这表明在对细菌、壳聚糖共轭物和侵染疫霉菌的处理反应中，保护性蛋白质的合成有多种调节机制。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.