Co-digestion of filter cake, biogas effluent, and anaerobic sludge for hydrogen and methane production: Optimizing energy recovery through two-stage anaerobic digestion

Abstract

This study assesses a two-stage anaerobic digestion process designed to efficiently recover energy through hydrogen and methane production by co-digesting filter cake (FC), biogas effluent (BE), and anaerobic sludge (AS) from the sugar and ethanol industry. The optimal proportions of FC, BE, and AS were determined in batch fermentation experiments using Design Expert software, which identified a suitable ratio of 31.05:28.95:0.00 (g VS/L), respectively. The results indicated that hydrogen production in the first stage could occur solely through the hydrogenic bacteria present in the BE and FC mixture, without the need for AS as an inoculum. This optimized FC:BE ratio was then applied in a semi-continuous fermentation system, achieving a hydrogen production rate of 193.6 mL H₂/L.d and a hydrogen yield of 9.8 mL H₂/g VS at an optimal hydraulic retention time (HRT) of 3 d. In the subsequent second stage, the effluent from the hydrogen reactor was used for methane production. This stage achieved a methane production rate of 422.0 mL CH₄/L·d and a methane yield of 140.2 mL CH₄/g VS, with an HRT of 20 d. Overall, the two-stage process exhibited an impressive energy output, peaking at 5.17 kJ/g VS. This suggests the potential for an annual electricity generation of 194,655 MWh and an estimated reduction of 85,668 tCO₂eq/year in greenhouse gas emissions. This study highlights the efficiency of the two-stage anaerobic digestion process for harnessing energy through the co-digestion of FC and BE, without the need for additional AS inoculum. The findings demonstrate the potential for sustainable energy recovery from industrial waste streams while mitigating environmental impacts.