The Effects of Plant Growth-promoting Bacteria on Seed Germination and Seedling Growth in Crassocephalum rubens

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-05 DOI:10.1139/cjb-2024-0005

Jr-Chi Ou, Yu-Heng Lin, Hyungmin Rho

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Abstract

Crassocephalum rubens, an herb with potential anticancer properties, encounters challenges in production efficiency due to small seed size and elevated temperatures in cultivation areas. Our study investigates the effects of seed pelleting and plant growth-promoting bacteria (PGPB) on germination and seedling growth across temperature variations in Taiwan. Pelleted seeds demonstrate superior germination percentages, linked to enhanced seed quality. Elevated temperature, particularly at 30/25°C, enhanced germination performance, with the highest final germination percentage observed in pelleted seeds treated with water. Unexpectedly, PGPB strains – Klebsiella sp. KsGRB10 and Bacillus sp. BsGFB04 – exhibited limited impact on germination rates, showing a marginal increase in seedling growth under 25/20°C and 30/25°C, respectively. Air temperature fluctuations influenced seedling vigor, leaf color, and physiological parameters. Remarkably, inoculation with BsGFB04 and KsGRB10 enhanced C. rubens’ tolerance to high-temperature stress conditions. Diurnal measurements in week 4, under 25/20°C, reveal that PGPB inoculation decreased stomatal conductance and transpiration rate while maintaining the quantum yield of PSII, indicating potentially improved water-use efficiency. This study provides crucial insights into the interplay among PGPB, environmental stress, and the physiology of a wild species, paving the way for further research in the domestication of C. rubens for medicinal herb mass production.

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植物生长促进菌对红豆杉种子萌发和幼苗生长的影响

红豆杉（Crassocephalum rubens）是一种具有潜在抗癌功效的草本植物，由于种子较小和种植区温度较高，其生产效率面临挑战。我们的研究调查了粒化种子和植物生长促进菌（PGPB）在台湾不同温度下对发芽和幼苗生长的影响。粒化种子的发芽率更高，这与种子质量的提高有关。温度升高，尤其是在 30/25°C 温度下，可提高发芽率，经水处理的丸粒种子的最终发芽率最高。出乎意料的是，PGPB 菌株--克雷伯氏菌 KsGRB10 和芽孢杆菌 BsGFB04--对发芽率的影响有限，在 25/20°C 和 30/25°C 温度下分别显示出幼苗生长的微弱增长。气温波动影响幼苗活力、叶色和生理参数。值得注意的是，接种 BsGFB04 和 KsGRB10 增强了红叶石楠对高温胁迫条件的耐受性。第 4 周在 25/20°C 条件下进行的昼夜测量显示，接种 PGPB 降低了气孔导度和蒸腾速率，同时保持了 PSII 的量子产率，这表明水分利用效率可能有所提高。这项研究为了解 PGPB、环境胁迫和野生物种生理机能之间的相互作用提供了重要见解，为进一步研究驯化 C. rubens 以用于药材大规模生产铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.