Characteristics of Clinical Isolates of Listeria monocytogenes in Sichuan, China, in 2022 Based on Whole Genome Sequencing Analysis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-10 DOI:10.1089/fpd.2023.0173

Qian Liang, Weifeng Huang, Tao Xiao, Lin Zhang, Gaopeng Lei, Hong Lv, Xiaorong Yang

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Abstract

Listeria monocytogenes is a foodborne pathogen. In 2022, we collected 15 strains of L. monocytogenes isolated from patients in some foodborne disease sentinel monitoring hospitals in Sichuan Province. Through whole genome sequencing (WGS), we obtained the virulence genes carried by the strains, multi-locus sequence typing (MLST), core genome MLST (cgMLST), clonal complex (CC), and serum groups and constructed a phylogenetic tree and minimum spanning tree with nonhuman strains. An analysis shows that all 15 strains of L. monocytogenes carry virulence genes LIPI-1 and LIPI-2, whereas the carrying rates of LIPI-3 and LIPI-4 virulence genes are relatively low. The MLST typing results showed a total of 10 sequence types (ST), including 10 CCs, with ST7 being the dominant type. The cgMLST clearly distinguishes strains of different lineages and CC types. The serum group is divided into three types: IIa, IIb, and IVb, with IIa being the dominant serum group. An analysis of antibiotic genes showed that all 15 strains carried FosX, lin, mprF, and norB with high carrying rates. The minimum inhibitory concentration results indicated that all were susceptible to eight antibiotics (ampicillin, penicillin, tetracycline, meropenem, erythromycin, vancomycin, ciprofloxacin, and trimethoprim-sulfamethoxazole). The analysis of strains isolated from different sources of Listeria revealed varying degrees of diversity, and the contamination of meat and environment within the province is closely related to clinical cases. L. monocytogenes isolated from clinical cases in Sichuan Province carry multiple virulence and antibiotic genes, with high potential pathogenicity. It is necessary to further strengthen the monitoring and control of food and environment by L. monocytogenes within Sichuan Province.

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基于全基因组测序分析的 2022 年中国四川李斯特菌临床分离菌特征

单核细胞增生李斯特菌是一种食源性致病菌。2022 年，我们从四川省一些食源性疾病哨点监测医院的患者中采集了 15 株单核细胞增生李斯特菌。通过全基因组测序（WGS），我们获得了这些菌株携带的毒力基因、多焦点序列分型（MLST）、核心基因组MLST（cgMLST）、克隆复合体（CC）和血清群，并构建了与非人类菌株的系统发生树和最小跨度树。分析表明，15 株单核细胞增多性酵母菌均携带毒力基因 LIPI-1 和 LIPI-2，而 LIPI-3 和 LIPI-4 毒力基因的携带率相对较低。MLST 分型结果显示共有 10 种序列类型（ST），包括 10 种 CC，其中 ST7 为优势类型。cgMLST 可以清楚地区分不同品系的菌株和 CC 类型。血清组分为三种类型：血清组分为三类：IIa、IIb 和 IVb，其中 IIa 为优势血清组。抗生素基因分析表明，所有 15 株菌株均携带 FosX、lin、mprF 和 norB，且携带率较高。最低抑菌浓度结果显示，所有菌株都对八种抗生素（氨苄西林、青霉素、四环素、美罗培南、红霉素、万古霉素、环丙沙星和三甲氧苄氨嘧啶-磺胺甲噁唑）敏感。对从李斯特菌不同来源分离到的菌株进行的分析表明，李斯特菌具有不同程度的多样性，该省的肉类和环境污染与临床病例密切相关。从四川省临床病例中分离出的单核细胞增生李斯特菌携带多种毒力基因和抗生素基因，具有较高的潜在致病性。有必要进一步加强对四川省食品和环境中单核细胞增生梭菌的监测和控制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.